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Optimization of Extraction of Cannabinoids and Flavonoids Substances from Influence Cannabis sp. and their Biological Activities before and after Decarboxylation

Olga Kronusová1, Michala Piklová1, Kristýna Šírová2, Petr Kaštánek2, Robert Gürlich3 and Petr Braťka4
  • 1 Department of Biotechnology UCT Prague, Faculty of Food and Biochemical Technology, Technická 3, 166 28 Prague, Czech Republic
  • 2 EcoFuel Laboratories Ltd., Ocelářská 9, 190 00 Praha, Czech Republic
  • 3 Surgical Clinic of the 3rd Faculty of Medicine of Charles University and the Royal Vinohrady University Hospital, Šrobárova 1150/50, 100 34 Praha 10, Czech Republic
  • 4 ING MEDICAL s.r.o., Praha 5 - Lahovice, K Sádkám 158, 159 00, Czech Republic

Abstract

Inflorescence of eight different varieties of Cannabis sp. (3 hemp varieties and 5 medical cannabis varieties) were subjected to extraction with n-hexane or ethanol as representatives of non-polar and polar solvents. Crude extracts were either used directly or after decarboxylation of cannabis acids for measurement of selected biological activities - cytotoxicity against carcinogenic line B16 (musculus skin melanoma cells), cytotoxicity against Human Dermal Fibroblast (HDF) as a control non-carcinogenic line, antimicrobial activity against Candida albicans (DBM, 2186), Staphylococcus aureus (ATCC, 25923) and Salmonella enterica (CCM, 4420) and antioxidant activity using the ORAC (Oxygen Radical Absorbance Capacity) assay. 32 samples with a different profile of cannabinoids measured by HLPC were thus obtained and evaluated. The content of total flavonoids that could exert synergic action on bioactivity was measured by the spectrophotometric method. The hexane extracts showed higher antimicrobial activity, decarboxylation of the samples led to an increase in cytotoxicity against the human skin fibroblast line in most of the studied samples, and to decrease in the antioxidant effects of the decarboxylated extracts. The obtained findings suggest a therapeutic potential of the selected cannabis extracts in the treatment of skin cancer, skin wounds, or surgical wound healing.

American Journal of Applied Sciences
Volume 19 No. 1, 2022, 68-77

DOI: https://doi.org/10.3844/ajassp.2022.68.77

Submitted On: 3 January 2022 Published On: 26 May 2022

How to Cite: Kronusová, O., Piklová, M., Šírová, K., Kaštánek, P., Gürlich, R. & Braťka, P. (2022). Optimization of Extraction of Cannabinoids and Flavonoids Substances from Influence Cannabis sp. and their Biological Activities before and after Decarboxylation. American Journal of Applied Sciences, 19(1), 68-77. https://doi.org/10.3844/ajassp.2022.68.77

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Keywords

  • Cannabinoids
  • Flavonoids
  • Cannabis Sativa
  • Entourage Effect
  • Cytotoxicity
  • Antimicrobial Effect
  • Antioxidant Activity