Research Article Open Access

Extra-Bone Marrow Sites of Haemopoeisis: Dihydroartemisinin Effects in Wistar Albino Rats

Nedosa Uchechukwu Anastasia1, Saleh Mohammed2, Nedosa Kenechi Stanislaus3, Njoku Goddy4, Ikenna Kenneth Onyedibe5, P. A. Akah6 and C. O. Okoli6
  • 1 Department of Pharmacology and Toxicology, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, P.M.B. 5025, Awka, Anambra State, Nigeria
  • 2 Department of Biochemistry Federal, College of Veterinary and Medical Laboratory Technology, Vom, Plateau State, Nigeria
  • 3 Evangelical Churches of West Africa (ECWA) Hospital, Egbe, Kogi State, Nigeria
  • 4 Medical Instructional Technology Unit, Jos University Teaching hospital, University of Jos, P.M.B. 2076, Jos, Plateau State, Nigeria
  • 5 Department of Medical Microbiology, Jos University Teaching Hospital, University of Jos, P.M.B. 2076, Jos, Plateau State, Nigeria
  • 6 Department of Pharmacology and Toxicology, University of Nigeria, Nsukka, Enugu State, Nigeria

Abstract

Problem statement: Iron is present in ferritin, the storage form of iron in the tissues; in the respiratory cytochrome enzymes; in hemoglobin of the blood and in the myoglobin of muscle Dihydroartemisinin (DHA) has been shown to interact with heme groups in vivo and in vitro. This study investigated the effects of 5 day and 7day oral dihydroartemisinin treatments on the blood and tissues of the lungs, the heart, the liver, the intestines, the spleen and the kidney of Wistar albino rats. The dosage regimens of dihydroartemisinin employed in the study were: A single dosage regimen of 1 mg kg-1; a repeated dosage regimen of 1; 2; 60 and 80 mg kg-1. Approach: The results of the study showed that dihydroartemisinin interacted with the hemoglobin of the blood and the myoglobin of muscle to stimulate new haemopoesis in a concentration, repetition and time dependent manner in the tissues of the lungs, liver, spleen, intestine, heart and kidney of Wistar albino rats which was absent in the control rats. Results: Statistically significant increases were observed in the Packed Cell Volume (PCV) (P<0.01-03) and the total White blood cell count (P<0.01) of the DHA-treated rats but not in the control rats. It also stimulated hyperplasia of erythrocyte and leucocyte stem cells in the lungs, liver, intestine, spleen, heart and kidney of DHA-treated Wistar albino rats not seen in the controls. Conclusion: These haemopoetic effects of DHA were greater and of longer duration in 5 day DHA-treatment rats than in those of the 7 day DHA-treatment rats.

American Journal of Applied Sciences
Volume 9 No. 1, 2012, 7-12

DOI: https://doi.org/10.3844/ajassp.2012.7.12

Submitted On: 23 August 2011 Published On: 2 November 2011

How to Cite: Anastasia, N. U., Mohammed, S., Stanislaus, N. K., Goddy, N., Onyedibe, I. K., Akah, P. A. & Okoli, C. O. (2012). Extra-Bone Marrow Sites of Haemopoeisis: Dihydroartemisinin Effects in Wistar Albino Rats. American Journal of Applied Sciences, 9(1), 7-12. https://doi.org/10.3844/ajassp.2012.7.12

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Keywords

  • Dihydroartemisinin (DHA)
  • bone marrow
  • wistar albino rats
  • haemopoetic effects
  • dosage regimen
  • blood cells
  • Packed Cell Volume (PCV)
  • Blood Cell Count (WBC)