Research Article Open Access

Validated High Performance Liquid Chromatographic Method for Analysis of Fenvalerate Pesticide in Chilies by QuEChERS Extraction Cleanup and High Liquid Chromatography

Ahmed Ambarak Altorshani1, Nor Kartini Abu Bakar1 and Eltayeb Elamin M. Eid2
  • 1 Department of Chemistry, Faculty of Science, University Malaya, Malaysia, 50603 Kuala Lumpur, Malaysia
  • 2 Laboratory of immunotherapeutic and vaccines, University Putra Malaysia, Serdang 43400, Selangor DE, Malaysia

Abstract

Problem statement: Contamination associated with pesticide use has increased as well, adversely impacting the environment and causing human health risks through residues on food. Approach: This study reports the extraction of Fenvalerate (FE) in chilies using QuEChERS based on High Performance Liquid Chromatography (HPLC) describes a simple, fast and inexpensive method. The chillie was extracted with acetonitrile Aliquots were cleaned-up using Solid Phase Extraction (dSPE), a primary-secondary amine carbon black. Reversed-phased HPlc system with PDA detection was used for the separation identification and quantification of all these analyses using acetonitrile methanol-potassium dehydrogenate phosphate (50:40:10) as mobile phase. Results: Limit of detection of 0.01 µg mL was obtained. Calibration curves that constructed for the analyzes spiked into samples followed linear relationships with good correlation coefficients (R2>0.9944). In this method was found to be precise, specific and accurate for detection and analysis of Fenvalerate in chilies. Conclusion: QuEChERS methods are convenient, rugged methods that simplify extract cleanup, reduce material costs and improve sample throughout. Here we demonstrate the effectiveness of QuEChERS sample cleanup using a multiresidue analysis of pesticide on chilies.

American Journal of Applied Sciences
Volume 7 No. 12, 2010, 1587-1592

DOI: https://doi.org/10.3844/ajassp.2010.1587.1592

Submitted On: 19 October 2010 Published On: 31 December 2010

How to Cite: Altorshani, A. A., Bakar, N. K. A. & Eid, E. E. M. (2010). Validated High Performance Liquid Chromatographic Method for Analysis of Fenvalerate Pesticide in Chilies by QuEChERS Extraction Cleanup and High Liquid Chromatography. American Journal of Applied Sciences, 7(12), 1587-1592. https://doi.org/10.3844/ajassp.2010.1587.1592

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Keywords

  • QuEChERS extraction
  • liquid chromatography
  • sulphate anhydrous
  • synthetic pyerthroid
  • dehydrogenate phosphate
  • Solid Phase Extraction (SPE)
  • fenvalerate pesticide