Research Article Open Access

Development of Growth Medium for Centella Asiatica Cell Culture Via Response Surface Methodology

Rozita Omar, M. A. Abdullah, M. A. Hasan and M. Marziah

Abstract

The effects of sucrose, Indole-3-Acetic Acid (IAA) and 6-BenzylAminoPurine (BAP) concentrations on cell growth of Centella asiatica cell suspension culture were studied. The concentrations were designed using Central-composite experimental design and regression analysis was carried out to obtain response surface model describing cell growth for prediction of optimum conditions. Only sucrose as a single factor was positively significant for cell growth. Increasing sucrose concentration from 3.32 to 6.68% (w/v) resulted in an increase in dry cell weight from 16-27 g L-1. IAA and BAP as single factors and other possible interaction effect were insignificant. The optimum values predicted to be 6.68% (w/v) sucrose, 0.84 mg L-1 IAA and 1.17 mg L-1 BAP yielding 27.4 g L-1 dry cell weight with 81.4% regression equation fitness of the experimental data.

American Journal of Applied Sciences
Volume 1 No. 3, 2004, 215-219

DOI: https://doi.org/10.3844/ajassp.2004.215.219

Submitted On: 10 March 2005 Published On: 30 September 2004

How to Cite: Omar, R., Abdullah, M. A., Hasan, M. A. & Marziah, M. (2004). Development of Growth Medium for Centella Asiatica Cell Culture Via Response Surface Methodology. American Journal of Applied Sciences, 1(3), 215-219. https://doi.org/10.3844/ajassp.2004.215.219

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Keywords

  • Central Composite
  • Centella asiatica
  • Plant Growth Regulator
  • Response Surface Methodology Sucrose