Research Article Open Access

Inulinase Production by Penicillium citrinum ESS in Submerged and Solid-State Cultures

Cynthia Lafuente-Castaneda1, Gabriela Martinez1, Juan C. Contreras-Esquivel1, Raul Rodriguez-Herrera1, Arely Prado-Barragan2 and Cristobal N. Aguilar1
  • 1 Department of Food Science and Technology, School of Chemistry, Universidad Autonoma de Coahuila. Blvd. Venustiano Carranza. Col. Republica, CP 25280, Saltillo, Coahuila, Mexico
  • 2 Departament of Biotechnology, Universidad Autonoma Metropolitana. Unidad Iztapalapa, CP 09340, D.F, Mexico

Abstract

Problem statement: This work reports the Penicillium citrinum ESS strain as a new source of inulinase (2,1-β-D fructanhydrolase, E.C. 3.2.1.7). Approach: The enzyme was produced in both, Solid-State (SSC) and Submerged Culture (SmC) using a basal medium added either with inulin or sucrose (5 gL-1). Results: The culture grew faster in SmC than in SSC with both carbon sources; however, yields of inulinase activity per gram of biomass were higher in SSC (1,658,237.28 for inulin and 66598.96 for sucrose) than in SmC (441.05 for inulin and 183.91 for sucrose). Conclusion: The highest inulinase production was obtained by SSC, either with inulin or sucrose, reaching values of 6650 and 2970 U/L respectively. In contrast, in SmC were obtained higher levels of inulinase activity (2241 U/L) with sucrose as carbon source (24 h), while with inulin, the enzyme production was negatively affected and retarded.

American Journal of Agricultural and Biological Sciences
Volume 7 No. 2, 2012, 129-134

DOI: https://doi.org/10.3844/ajabssp.2012.129.134

Submitted On: 13 November 2011 Published On: 13 March 2012

How to Cite: Lafuente-Castaneda, C., Martinez, G., Contreras-Esquivel, J. C., Rodriguez-Herrera, R., Prado-Barragan, A. & Aguilar, C. N. (2012). Inulinase Production by Penicillium citrinum ESS in Submerged and Solid-State Cultures. American Journal of Agricultural and Biological Sciences, 7(2), 129-134. https://doi.org/10.3844/ajabssp.2012.129.134

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Keywords

  • Penicillium citrinum ESS
  • inulinase production
  • Solid-State Culture (SSC)