@article {10.3844/ajabssp.2012.129.134, article_type = {journal}, title = {Inulinase Production by Penicillium citrinum ESS in Submerged and Solid-State Cultures}, author = {Lafuente-Castaneda, Cynthia and Martinez, Gabriela and Contreras-Esquivel, Juan C. and Rodriguez-Herrera, Raul and Prado-Barragan, Arely and Aguilar, Cristobal N.}, volume = {7}, year = {2012}, month = {Mar}, pages = {129-134}, doi = {10.3844/ajabssp.2012.129.134}, url = {https://thescipub.com/abstract/ajabssp.2012.129.134}, abstract = {Problem statement: This work reports the Penicillium citrinum ESS strain as a new source of inulinase (2,1-β-D fructanhydrolase, E.C. 3.2.1.7). Approach: The enzyme was produced in both, Solid-State (SSC) and Submerged Culture (SmC) using a basal medium added either with inulin or sucrose (5 gL-1). Results: The culture grew faster in SmC than in SSC with both carbon sources; however, yields of inulinase activity per gram of biomass were higher in SSC (1,658,237.28 for inulin and 66598.96 for sucrose) than in SmC (441.05 for inulin and 183.91 for sucrose). Conclusion: The highest inulinase production was obtained by SSC, either with inulin or sucrose, reaching values of 6650 and 2970 U/L respectively. In contrast, in SmC were obtained higher levels of inulinase activity (2241 U/L) with sucrose as carbon source (24 h), while with inulin, the enzyme production was negatively affected and retarded.}, journal = {American Journal of Agricultural and Biological Sciences}, publisher = {Science Publications} }