Research Article Open Access

Screening of Pseudomonas sp. Isolated from Rhizosphere of Soybean Plant as Plant Growth Promoter and Biocontrol Agent

Aris Tri Wahyudi1, Rika Indri Astuti1 and Giyanto2
  • 1 Department of Biology, Faculty of Mathematics and Natural Science, Bogor Agricultural University, Jl. Agatis-Kampus IPB Darmaga, Bogor 16680, Indonesia
  • 2 Department of Plant Protection, Faculty of Agriculture, Bogor Agricultural University, Jl. Agatis-Kampus IPB Darmaga, Bogor 16680, Indonesia

Abstract

Abstract: Problem statement: Pseudomonas spesies are one of the rihizobacterial group that have an important role in plant growth promoter and plant health. To prepare them as inoculants, they must have a range of characters as growth promoter such as Indole Acetic Acid (IAA) producers which can promote the growth of plants and solubilize phosphates. In addition, they must also have the various characters that act as biocontrol agents such as siderofor, chitinase and anti-fungal compound producers. Approach: Pseudomonas sp isolated from soybeans rhizospere and identified based on physiological reactions and 16S rRNA gene sequences. Various tests for the determination of the growth promoter were based on IAA production, phosphate solubilization and growth promoter of length of root and stems and number of lateral roots of soybean sprouts. Test of siderophore, chitinase, as well as anti anti-fungal compounds productions to inhibit the growth of Fusarium oxysporum, Rhizoctonia solani and Sclerotium rolfsii, were used as a biocontrol agent determination. Hypersensitivity test was used to screen for Pseudomonas sp classified as non-pathogenic rhizobacteria. Results: Fourteen isolates identified as a non-pathogenic Pseudomonas sp that produced IAA and Promoted enhancement of root length, shoot length, or number of lateral root. Among those 14 isolates, 8 isolates showed phosphate solubilizing activity, 12 isolates capable of producing siderophore and six isolates were observed to have chitinolytic activity. Only three isolates were able to inhibit the growth of Fusarium oxysporum in high level. While one and two isolates inhibited Sclerotium rolfsii and Rhizoctonia solani in high level, respectively. Conclusion: On the basis of excellent growth promoter and biocontrol activities, we recommended 5 isolates of Pseudomonas sp which were Crb-3, Crb-16, Crb-17, Crb-44 and Crb-94 as potential isolates of Pseudomonas sp that could be applied as inoculants of soybean plant.

American Journal of Agricultural and Biological Sciences
Volume 6 No. 1, 2011, 134-141

DOI: https://doi.org/10.3844/ajabssp.2011.134.141

Submitted On: 30 December 2010 Published On: 10 March 2011

How to Cite: Wahyudi, A. T., Astuti, R. I. & Giyanto, (2011). Screening of Pseudomonas sp. Isolated from Rhizosphere of Soybean Plant as Plant Growth Promoter and Biocontrol Agent. American Journal of Agricultural and Biological Sciences, 6(1), 134-141. https://doi.org/10.3844/ajabssp.2011.134.141

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Keywords

  • Rhizobacteria
  • Pseudomonas sp
  • growth promoter
  • biocontrol
  • soybean
  • Indole Acetic Acid (IAA)
  • Plant Growth Promoting Rhizobacteria (PGPR)
  • Analysis Of Variance (ANOVA)
  • Solubilizing Index (SI)
  • Phosphate Solubilizing Bacteria (PSB)
  • Induced Systemic Resistance (ISR)
  • rRNA gene