TY  - JOUR
AU  - Wahyudi, Aris Tri 
AU  - Astuti, Rika Indri 
AU  - Giyanto, 
PY  - 2011
TI  - Screening of Pseudomonas sp. Isolated from Rhizosphere of Soybean Plant as Plant Growth Promoter and Biocontrol Agent
JF  - American Journal of Agricultural and Biological Sciences
VL  - 6
IS  - 1
DO  - 10.3844/ajabssp.2011.134.141
UR  - https://thescipub.com/abstract/ajabssp.2011.134.141
AB  - Abstract: Problem statement: Pseudomonas spesies are one of the rihizobacterial group that have an
important role in plant growth promoter and plant health. To prepare them as inoculants, they must
have a range of characters as growth promoter such as Indole Acetic Acid (IAA) producers which can
promote the growth of plants and solubilize phosphates. In addition, they must also have the various
characters that act as biocontrol agents such as siderofor, chitinase and anti-fungal compound
producers. Approach: Pseudomonas sp isolated from soybeans rhizospere and identified based on
physiological reactions and 16S rRNA gene sequences. Various tests for the determination of the
growth promoter were based on IAA production, phosphate solubilization and growth promoter of
length of root and stems and number of lateral roots of soybean sprouts. Test of siderophore, chitinase,
as well as anti anti-fungal compounds productions to inhibit the growth of Fusarium oxysporum,
Rhizoctonia solani and Sclerotium rolfsii, were used as a biocontrol agent determination.
Hypersensitivity test was used to screen for Pseudomonas sp classified as non-pathogenic
rhizobacteria. Results: Fourteen isolates identified as a non-pathogenic Pseudomonas sp that produced
IAA and Promoted enhancement of root length, shoot length, or number of lateral root. Among those
14 isolates, 8 isolates showed phosphate solubilizing activity, 12 isolates capable of producing
siderophore and six isolates were observed to have chitinolytic activity. Only three isolates were able
to inhibit the growth of Fusarium oxysporum in high level. While one and two isolates inhibited
Sclerotium rolfsii and Rhizoctonia solani in high level, respectively. Conclusion: On the basis of
excellent growth promoter and biocontrol activities, we recommended 5 isolates of Pseudomonas sp
which were Crb-3, Crb-16, Crb-17, Crb-44 and Crb-94 as potential isolates of Pseudomonas sp that
could be applied as inoculants of soybean plant.