Comparison of IncL/M Plasmids Using the Neighbor-Joining Method on Basis repA and excA Genes
Touati Abdelaziz and Mairi Assia
DOI : 10.3844/ajbsp.2019.14.17
Current Research in Bioinformatics
Volume 8, 2019
The aim of this study is to make comparison of IncL/M groups on basis of two genes candidates’ repA and excA genes. The sequences of 27 plasmids were compared using the neighbor-joining method. This method was used to construct a phylogenetic tree for the nucleotide sequences of two genes (repA and excA), using the program MEGA X software. The evolutionary distances were computed using the maximum composite likelihood method. The neighbor-joining method analysis showed different results based on the gene used for comparison. The repA gene was more accurate than excA gene to distinguish between different incL/M plasmids. This study suggested that IncL/M plasmids harboring different antibiotic resistance genes have evolved differently.
© 2019 Touati Abdelaziz and Mairi Assia. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.