Synthesis and Biological Evaluation of 6-Hydroxy-4-Methyl-5,7-(Bis-p-ChlorophenylAzo) Coumarin
Dalal M. Ibrahim, Juliana Jumal and Farah Wahida Harun
DOI : 10.3844/ajassp.2016.1.6
American Journal of Applied Sciences
Volume 13, Issue 1
6-hydroxy-4-methyl-5,7-(bis-p-chlorophenylazo)coumarinhas been synthesized and characterizedby CHN elemental analysis, FTIR, 1H-NMR-spectroscopy andmass-spectral data. Cytotoxic screening by MTT assay was carried out on the compoundagainst breast cancer cells. The overall results from preliminary screeningprogram revealed that the cell proliferation was highly inhibited by 6-hydroxy-4-methyl-5,7-(bis-p-chlorophenylazo)coumarin with the value of 2.81%, at concentration of 30 µg mL-1 comparedwith the untreated control cells and also possessed a good chelating activitywith IC50 value 1.87 µg mL-1. It issuggested that the cytotoxic activity is affected by hydroxyl and halogengroups as these groups have high electron affinity and high electronegativity.
© 2016 Dalal M. Ibrahim, Juliana Jumal and Farah Wahida Harun. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.