Screening of Lactic Acid Bacteria for Antifungal Activity against Aspergillus oryzae
Belal Jamal Muhialdin and Zaiton Hassan
DOI : 10.3844/ajassp.2011.447.451
American Journal of Applied Sciences
Volume 8, Issue 5
Problem statement: The growth of spoilage fungi have been a global concern because of the economy loses and the health hazard of the mycotoxins produced by the spoilage fungi. Approach: A total of 137 lactic acid bacteria isolated from Malaysian fruits and fermented foods were screened for antifungal activity using dual agar overlay method and well method against A. oryzae. Results: 23 isolates showed inhibition activity after 72 h incubation at 30°C. Supernatant of three isolates with strong antifungal activity was evaluated by well method and they inhibited the growth of the fungi at 30°C for 72 h. LAB supernatant reduced the mass growth of Aspergillus oryzae when incubated for 7 days at 30°C. The isolates were identified using API 50CH as Lactobacillus brevis G004, Lactobacillus fermentum Te007 and Pediococcus pentosaceus Te010. Conclusion: The three isolates studied inhibited the growth of the mycelia and conidia germination of the fungi which indicate the possibility of using LAB isolates as biopreservative.
© 2011 Belal Jamal Muhialdin and Zaiton Hassan. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.