The Effect of Physical Refining on Palm Vitamin E (Tocopherol, Tocotrienol and Tocomonoenol)
Chiew Wei Puah, Yuen May Choo, Ah Ngan Ma and Cheng Hock Chuah
DOI : 10.3844/ajassp.2007.374.377
American Journal of Applied Sciences
Volume 4, Issue 6
This study reports the effect of physical refining on palm vitamin E including Î±âtocopherol, Î±â, γâ and Î´âtocotrienols as well as Î±âtocomonoenol. A method using HPLC with fluorescence detector using normal phase silica column is described. An isocratic elution with n-hexane/THF/2-propanol (1000:60:4, by vol.) as mobile phase was used. The structure of the Î±âtocomonoenol was determined using gas chromatography coupled with mass spectrometry. The composition of the vitamers were Î±âtocopherol (14â17%), Î±âtocotrienol (22â24%), γâtocotrienol (49â53%), Î´âtocotrienol (6â7%) and Î±âtocomonoenol (3%) throughout the physical refining. The concentration of all vitamers in crude palm oil was 1273Â±18 ppm. The concentrations of all vitamers in degummed palm oil, bleached palm oil and deodorized palm oil were 1134Â±20 ppm, 1095Â±18 ppm and 1029Â±18 ppm, respectively. This method provides fast and valuable information with minimal analysis time and no sample pre-treatment.
© 2007 Chiew Wei Puah, Yuen May Choo, Ah Ngan Ma and Cheng Hock Chuah. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.