Separation of Coenzyme Q10 in Palm Oil by Supercritical Fluid Chromatography
Ng M. Han, Choo Y. May, Ma A. Ngan, Chuah C. Hock and Mohd A. Hashim
DOI : 10.3844/ajassp.2006.1929.1932
American Journal of Applied Sciences
Volume 3, Issue 7
Palm oil is known to host a variety of phytonutrients; some having antioxidant property such as the carotenes and vitamin E. These antioxidants are also present in the oil recovered from the palm-pressed fibre. Study was carried out to investigate the presence of coenzyme Q10, yet another non-glyceride compound which possesses antioxidant property in crude palm oil (CPO) and palm fibre oil. Separation of coenzyme Q10 in CPO and palm fibre oil was carried out using supercritical fluid chromatography with ultra violet detection. 1H and 13C NMR were used for its characterisation. Calibration with authentic standard shows that there are 10-80 ppm coenzyme Q10 in CPO while its concentration in palm fibre oil is 1000-1500 ppm.
© 2006 Ng M. Han, Choo Y. May, Ma A. Ngan, Chuah C. Hock and Mohd A. Hashim. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.