Gastroprotective Effects of β-sitosterol and β-sitosterol-3-O-β-D-glucopyranoside from Bridelia ferruginea Stem Bark
- 1 University of Nigeria, Nigeria
- 2 University of Nigeria, Nsukka, Nigeria
Copyright: © 2021 Michel Tchimene Kenne, Adaobi Chioma Ezike, Charles Ogbonnaya Okoli, Peter Achunike Akah and Ejike Marcellus Nnamani. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The methanol extract of Bridelia ferruginea Benth. (Euphorbiaceae) stem bark (BFME) was partitioned in chloroform-methanol-water (2:2:1) mixture to obtain the Chloroform (CF) and Aqueous Methanol (AMF) fractions. The BFME, CF and AMF were screened for antiulcer activity using indomethacin-induced ulcer as activity guide. The CF provided the highest gastroprotection and was subsequently fractionated in a silica gel (60-200 mesh) column eluted with different mixtures of n-hexane and ethyl acetate (100:0; 95:5; 90:10; 80:20) to obtain six fractions (I-VI). Fractions III and VI offered the highest protection against indomethacin-induced ulcer and were further purified in a sephadex LH-20 column eluted with methanol to yield two compounds, BF1 and BF2. Using Nuclear Magnetic Resonance (1H-NMR, 13C-NMR) and electron impact mass spectroscopies, BF1 and BF2 were confirmed to be β-sitosterol and β-sitosterol-3-O-βD glucopyranoside respectively. The BFME, fractions, β-sitosterol and β-sitosterol-3-O-β-D-glucopyranoside elicited dose-related and significant (P<0.05) protection against various ulcers in rats. β-sitosterol, 100 and 300 mg/kg, produced 79.70, 82.18, 42.31, 44.87, 65.97, 70.83, 80.22 and 87.91% gastroprotection; while β-sitosterol-3-O-β-D-glucopyranoside, 100 and 300 mg/kg, caused 69.80 and 74.26, 33.33, 35.26, 84.03, 95.83, 83.52 and 85.71% gastroprotection against indomethacin-, ethanol-, cold restraint stress- and pylorus ligation- induced ulcers, respectively. Results demonstrated gastroprotective effects of B. ferruginea stem bark, attributable to β-sitosterol and β-sitosterol-3-O-β-D-glucopyranoside.
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- Cold Restraint Stress Ulcer
- Indomethacin-Induced Gastric Ulcer
- Pyloric Ligation