@article {10.3844/ajptsp.2020.29.39,
article_type = {journal},
title = {Gastroprotective Effects of β-sitosterol and β-sitosterol-3-O-β-D-glucopyranoside from Bridelia ferruginea Stem Bark},
author = {Kenne, Michel Tchimene and Ezike, Adaobi Chioma and Okoli, Charles Ogbonnaya and Akah, Peter Achunike and Nnamani, Ejike Marcellus},
volume = {15},
year = {2021},
month = {Jan},
pages = {29-39},
doi = {10.3844/ajptsp.2020.29.39},
url = {https://thescipub.com/abstract/ajptsp.2020.29.39},
abstract = {The methanol extract of Bridelia ferruginea Benth. (Euphorbiaceae) stem bark (BFME) was partitioned in chloroform-methanol-water (2:2:1) mixture to obtain the Chloroform (CF) and Aqueous Methanol (AMF) fractions. The BFME, CF and AMF were screened for antiulcer activity using indomethacin-induced ulcer as activity guide. The CF provided the highest gastroprotection and was subsequently fractionated in a silica gel (60-200 mesh) column eluted with different mixtures of n-hexane and ethyl acetate (100:0; 95:5; 90:10; 80:20) to obtain six fractions (I-VI). Fractions III and VI offered the highest protection against indomethacin-induced ulcer and were further purified in a sephadex LH-20 column eluted with methanol to yield two compounds, BF1 and BF2. Using Nuclear Magnetic Resonance (1H-NMR, 13C-NMR) and electron impact mass spectroscopies, BF1 and BF2 were confirmed to be β-sitosterol and β-sitosterol-3-O-βD glucopyranoside respectively. The BFME, fractions, β-sitosterol and β-sitosterol-3-O-β-D-glucopyranoside elicited dose-related and significant (P},
journal = {American Journal of Pharmacology and Toxicology},
publisher = {Science Publications}
}