Research Article Open Access

Knock Down of Erythrulose Kinase (eyk1) Leads to the Enhancement of Erythritol Production in Yarrowia lipolytica Mutant Strain YE4-2

Xinhe Zhao1, Tianlong Huang2, Yaxin Zhang2, Xiaojie Ren3, Yuanda Song1 and Bei Guo4
  • 1 Shandong University of Technology, China
  • 2 Beijing University of Agriculture, China
  • 3 Baolingbao Biology Co. Ltd., China
  • 4 Ministry of Agriculture and Rural Affairs, China
American Journal of Biochemistry and Biotechnology
Volume 16 No. 3, 2020, 332-344

DOI: https://doi.org/10.3844/ajbbsp.2020.332.344

Submitted On: 3 June 2020
Published On: 24 July 2020

How to Cite: Zhao, X., Huang, T., Zhang, Y., Ren, X., Song, Y. & Guo, B. (2020). Knock Down of Erythrulose Kinase (eyk1) Leads to the Enhancement of Erythritol Production in Yarrowia lipolytica Mutant Strain YE4-2. American Journal of Biochemistry and Biotechnology, 16(3), 332-344. https://doi.org/10.3844/ajbbsp.2020.332.344

Abstract

Erythritol is a nearly zero-calorie sweetener, which is in great demand in food and health care industry. Our previous work obtained a Yarrowia lipolytica mutant strain YE4-2 with high erythritol yield. However, its conversion rate of glucose needs further improvement. Erythrulose kinase (eyk1) participates in the early stages of erythritol catabolism; the existence of eyk1 prevents the accumulation of erythritol. In this study, the genetic transformation system of Y. lipolytica YE4-2 was firstly established and the Cre/loxp homologous recombination knockout system was constructed. By using the Cre/loxp system, eyk1 gene was deleted. According to the PCR verification of eyk1 gene, it was preliminarily determined that one allele of eyk1 in this recombinant strain was knocked out. The recombination strain showed an average conversion rate of 48.51%, which was 49.26% higher than that of YE4-2 and the erythritol productivity is increased by 62.26%, which would be a significant improvement in the industrial scale.

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Keywords

  • Erythritol
  • Yarrowia lipolytica
  • Genetic Transformation System
  • Cre/loxp
  • eyk1