Research Article Open Access

In Vitro Callus Induction and Regeneration of Medicinal Plant Datura innoxia

Baan Munim Twaij1, Alaa Jabbar Taha1 and Md. Nazmul Hasan2
  • 1 Mustansiriyah University, Iraq
  • 2 Shahjalal University of Science and Technology, Bangladesh
American Journal of Biochemistry and Biotechnology
Volume 15 No. 3, 2019, 150-156

DOI: https://doi.org/10.3844/ajbbsp.2019.150.156

Published On: 17 August 2019

How to Cite: Twaij, B. M., Taha, A. J. & Hasan, M. N. (2019). In Vitro Callus Induction and Regeneration of Medicinal Plant Datura innoxia. American Journal of Biochemistry and Biotechnology, 15(3), 150-156. https://doi.org/10.3844/ajbbsp.2019.150.156

Abstract

The present study indicate the possibility of the development of callus from stem cut, leaf and root explants and regeneration of Datura plant (Datura innoxia) on Murashige and Skoog (MS) media supplemented with different concentrations of 6-Benzylaminopurine (BA) and 1-Naphthaleneacetic (NAA). Sterilized explants were inoculated and incubated in culture shade under different conditions (light and dark). Data analysis showed that maximum percentage (83%) of calli were induced from stem cut explants on MS media supplemented with NAA and BA at 0.5 and 1.0 mg/l respectively under dark condition. Maximum fresh and dry weight was found 395.63 mg and 35.64 mg respectively with supplementation of 0.5 mg/l NAA and 1 mg/l BA. Stem cut derived calli were transfer to MS regeneration medium supplementing different concentrations of plant regulators (PGRs) under different conditions. Maximum regeneration (78%) was found on MS media supplementing 1 mg/l of BA and 1 mg/l off NAA under dark condition. The attempt for callus induction from the explants (stem, leaf, root) using MS media with supplementation of different combinations of 2, 4-Dichlorophenoxyacetic acid (2, 4-D), BA and NAA, Kinetin (kin) under both light and dark conditions were carried out but no significant results were found.

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Keywords

  • Datura innoxia
  • Callus
  • Regeneration
  • 1-Naphthaleneacetic
  • 6-Benzylaminopurine