The -863 TNF-α Polymorphism and Primary Open Angle Glaucoma in Egyptians
Walaa Bayoumie Abdulwahhab El Gazzar, Shaymaa Mohamed Abd El Rahman, Mohamed Nagy Elmohamady, Ahmed Mohamed Saeed and Usama Shalaby
DOI : 10.3844/ajbbsp.2017.85.89
American Journal of Biochemistry and Biotechnology
Volume 13, Issue 2
Growing evidence indicates that TNF-alpha is involved in the pathogenesis of POAG in several ways, primarly by induction of retinal ganglion cells apoptosis and therefore optic nerve degeneration. TNF-alpha and POAG relationship has been studied at the genetic level with variable results in different populations. The transcription rate and the release of the TNF-alpha cytokine have been reported to be affected by polymorphisms in the promoter of the TNF- alpha gene. Polymorphisms at positions -238 and -308 are the most frequent studied. Another polymorphism, at the position -863 in the promoter region, has been less studied, but a homozygous AA allele appears protective in a Chinese population. Our aim was to assess the potential association of -863C/A TNF-alpha gene promoter polymorphisms with POAG in an Egyptian group of subjects. Genotyping of the TNF-alpha (-863) polymorphism was done for 228 POAG patients and 230 control subjects using the PCR-based, Restriction Fragment Length Polymorphism (RFLP) assay. TNF-alpha (-863) A/A genotype was absent in both groups. There was no significant difference between both groups as regards to TNF-α (-863) A allele carriage (6.14 versus 10.43%; p = 0. 099)). Also the genotype TNF-α (-863) C/C and the frequency of the tumor necrosis factor-alpha (-863) C allele did not significantly differ between both groups (93.86 versus 89.57%; p = 0. 099) and 96.93 versus 94.78%; p = 0. 107) respectively. Our data indicated that the TNF-alpha (-863) a allele is not linked with primary open angle glaucoma protection among Egyptian patients.
© 2017 Walaa Bayoumie Abdulwahhab El Gazzar, Shaymaa Mohamed Abd El Rahman, Mohamed Nagy Elmohamady, Ahmed Mohamed Saeed and Usama Shalaby. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.