American Journal of Biochemistry and Biotechnology

In silico Analysis of 4CL Family in Scutellaria baicalensis through Biocomputational Tools and Servers

Guoming Li, Xiaozhong Lan, Xiaorong Shui, Shian Huang, Can Chen and Wei Lei

DOI : 10.3844/ajbbsp.2017.27.33

American Journal of Biochemistry and Biotechnology

Volume 13, Issue 1

Pages 27-33

Abstract

The gene sequences of 4-coumarate:coenzyme A ligase (4CL) family of Scutellaria baicalensis came from the GenBank Database. With help of some bioinformatics tools such as Vector NTI Suite 8, ProtParam and SWISS-MODEL and so on, a series of biological information of their nucleic acid sequences and amino acid sequences were predicted and analyzed and the results were revealed as following: Two 4cl member genes share high similarity in structure and properties on level of nucleic acid and amino acid molecular. 4CLs are hydrophic proteins without any transmembrane topological structure and some crucial motifs were found. The secondary structures of Sb4CLs are mainly composed of random coil and α-helix and the models of their tertiary structures were built. In silico analysis of Sv4CL was finished, which would pave for further studies of physichemical properties of 4CL family and its related molecular mechanism of flavonoid metabolic regulation.

Copyright

© 2017 Guoming Li, Xiaozhong Lan, Xiaorong Shui, Shian Huang, Can Chen and Wei Lei. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.