American Journal of Biochemistry and Biotechnology

Simultaneous Detection of Nitrite and Nitrate in Celeries and Carrots

Dongwu Liu, Jinye Niu, Ling Kong and Zhiwei Chen

DOI : 10.3844/ajbbsp.2016.122.126

American Journal of Biochemistry and Biotechnology

Volume 12, Issue 2

Pages 122-126

Abstract

In this study, the anions nitrite ion (NO2-) and nitrate ion (NO3-) in celeries and carrots were detected with the technique of ion chromatography. A Dionex ICS-2000 ion chromatograph was used to detect the content of NO2- and NO3- in celeries and carrots. NO2- and NO3- were separated on a DIONEX Ionpac AS19 A-4 mm ion-exchange column and an Iopac AG AS19 A-4 mm guard column. The eluant was suppressed with an anion electrical self-regenerating suppressor (ASRS 300) and the content of NO2- and NO3- was detected with a conductivity detector. The detection limits were obtained for anions NO2- and NO3-. The present results showed that the ion chromatography was a suitable technique for the simultaneous determination of NO2- and NO3- in vegetables.

Copyright

© 2016 Dongwu Liu, Jinye Niu, Ling Kong and Zhiwei Chen. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.