American Journal of Biochemistry and Biotechnology

Amino Acid Sequence of Amylase Type Alpha, MiAmy, from Ok-Rong Mango (Mangifera indica Linn. cv. Ok-Rong)

Raksmont Ubonbal, Saijai Posoongnoen, Jureerut Daduang, Sompong Klaynongsruang and Sakda Daduang

DOI : 10.3844/ajbbsp.2015.119.126

American Journal of Biochemistry and Biotechnology

Volume 11, Issue 3

Pages 119-126

Abstract

The 594-amino acid residue sequence of α-amylase, MiAmy, from the Ok-rong mango (Mangifera indica Linn. cv. Ok-rong), in the ripening stage, was determined through Reverse Transcription Polymerase Chain Reaction (RT-PCR). Sequence alignments and evolutionary tree analyses revealed its high similarity to plastid α-amylase from many other plants. The sequence was revealed to have four conserved regions with catalytic amino acid residues for the active site. It was classified as a member of α-amylase family 13 because it has an active Domain A, similar to a (β/α)8-barrel structure. Three-dimensional structural predictions revealed that this partial sequence completely covered all of necessary domains for amylase activity.

Copyright

© 2015 Raksmont Ubonbal, Saijai Posoongnoen, Jureerut Daduang, Sompong Klaynongsruang and Sakda Daduang. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.