Research Article Open Access

CELL DETACHMENT BY PROLYL-SPECIFIC ENDOPEPTIDASE FROM WOLFIPORIA COCOS

Katharina Cierpka1, Nicole Mika2, Michaela C. Lange1, Holger Zorn2, Peter Czermak3 and Denise Salzig1
  • 1 University of Applied Sciences Mittelhessen, Germany
  • 2 Justus Liebig University Giessen, Germany
  • 3 Kansas State University, United States

Abstract

As requirements for Advanced Therapy Medicinal Product (ATMP) production differ from other production processes (e.g., therapeutic protein production), cell detachment is often a crucial step for the process success. In most cases, cell detachment is done enzymatically. Although many peptidases are established in cell culture in R&D, e.g., Trypsin as gold standard, many of them seem to be unsuitable in ATMP production processes. Therefore, the present study investigated a novel endopeptidase used in food biotechnology for its applicability in ATMP processes where cell detachment is needed. The Prolyl-specific Peptidase (PsP) is of non-mammalian origin and considered as safe for humans. PsP was purified from the supernatant of the fungus Wolfiporia cocos. The isolation and purification resulted in an enzyme solution with 0.19 U mg-1 prolyl-specific activity. By in silico analysis it was confirmed that attachment-promoting proteins can be cleaved by PsP in a similar amount than with Trypsin. Further the proteolytic activity was determined for PsP and Trypsin by using the same enzymatic assay. Detachment with both enzymes was compared for cells used in typical therapeutic production processes namely a mesenchymal stem cell line (hMSC-TERT) as a model for a cell therapeutic, Vero and MA104 cells used for viral therapeutic or vaccine production. The cell detachment experiments were performed with comparable enzyme activities (1.6 U mL-1). hMSC-TERT detachment was faster with PsP than with Trypsin. For Vero cells the detachment with PsP was not only faster but also more efficient. For MA104 cells the detachment rate with PsP was similar to Trypsin. For all cell types, detachment with PsP showed less influence on cell growth and metabolism compared to standard Trypsin.Thus, three cell types used in ATMP, viral therapeutics or vaccine production can be detached efficiently and gently with PsP. Therefore, PsP shows potential for cell detachment in ATMP and viral/vaccine production processes.

American Journal of Biochemistry and Biotechnology
Volume 10 No. 1, 2014, 14-21

DOI: https://doi.org/10.3844/ajbbsp.2014.14.21

Submitted On: 20 January 2014 Published On: 8 February 2014

How to Cite: Cierpka, K., Mika, N., Lange, M. C., Zorn, H., Czermak, P. & Salzig, D. (2014). CELL DETACHMENT BY PROLYL-SPECIFIC ENDOPEPTIDASE FROM WOLFIPORIA COCOS. American Journal of Biochemistry and Biotechnology, 10(1), 14-21. https://doi.org/10.3844/ajbbsp.2014.14.21

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Keywords

  • Cell Detachment
  • Prolyl-Specific Peptidase
  • ATMP
  • Trypsin
  • Wolfiporia cocos