Research Article Open Access

In Vivo Neutralization of Naja nigricollis Venom by Uvaria chamae

Omale James1, Ebiloma Unekwuojo Godwin1 and Idoko Grace Otini1
  • 1 Kogi State University, Nigeria
American Journal of Biochemistry and Biotechnology
Volume 9 No. 3, 2013, 224-234

DOI: https://doi.org/10.3844/ajbbsp.2013.224.234

Submitted On: 8 May 2012 Published On: 21 September 2013

How to Cite: James, O., Godwin, E. U. & Otini, I. G. (2013). In Vivo Neutralization of Naja nigricollis Venom by Uvaria chamae. American Journal of Biochemistry and Biotechnology, 9(3), 224-234. https://doi.org/10.3844/ajbbsp.2013.224.234

Abstract

Uvaria chamae is a well known medicinal plant in Nigerian traditional medicine for the management of many diseases, but investigations concerning its pharmacological characteristics are rare. In this study, we evaluate its venom neutralizing properties against Naja nigricollis venom in rats. Freshly collected Uvaria chamae leaves were air dried, powdered and extracted in methanol. To study the antivenom properties, albino rats were orally administered with a dose of 400 mg kg-1 body weight and one hour later, the venom was administered intraperitoneally at a dose of 0.08 mg kg-1 body weight of rats. Albino rats (male) weighing between 180-200g were randomly divided into five (5) groups of three (3). Groups 1-5 received water, normal saline, venom, Uvaria chamae and venom, Uvaria chamae respectively. Blood clothing time, bleeding time, antipyretic activity, haemoglobin, RBC, WBC, creatine kinase, AST, ALP and ALT activities total protein antioxidant activity and some blood electrolytes, plasma urea and uric acid were measured. Our results showed that Uvaria chamae methanol extract neutralized some biological effects of Naja nigricollis venom. The venom increased the rectal temperature, enzyme activities, bleeding time and other blood parameters. The plant extract was able to reduce these parameters in the extract treated groups. Details of the results are discussed. From this study, it is clear that U. chamae leaf extract had antivenom activity in animal models. The above results indicate that the plant extract possess potent snake venom neutralizing capacity and could potentially be used for therapeutic purpose in case of snake bite envenomation.

  • 1,292 Views
  • 2,497 Downloads
  • 4 Citations

Download

Keywords

  • Packed Cell Volume (PCV)
  • White Blood Cell (WBC)
  • Red Blood Cell (RBC)
  • U. chamae Methanol Extract Neutralized
  • Antipyretic Activity
  • Packed Cell Volume (PCV)