American Journal of Biochemistry and Biotechnology

The Effect of Citrullus colocynthis Pulp Extract on the Liver of Diabetic Rats a Light and Scanning Electron Microscopic Study

Mohammad Khalil, Gamal Mohamed, Mohammad Dallak, Fahaid Al-Hashem, Hussein Sakr, Refaat A. Eid, Mohamed A. Adly, Mahmoud Al-Khateeb, Saleh Banihani, Zuhair Hassan and Nabil Bashir

DOI : 10.3844/ajbbsp.2010.155.163

American Journal of Biochemistry and Biotechnology

Volume 6, Issue 3

Pages 155-163

Abstract

Problem statement: The goal of the current investigation was to clarify the effects of Citrullus colocynthis pulp extract on the structure of the liver of diabetic rats at both light and scanning electron microscopic levels. Approach: Forty-eight adult male albino rats were equally allocated into four groups: Group1: control, Group 2: Citrullus colocynthis-treated, Group 3: diabetic rats and Group4: diabetic rats treated with Citrullus colocynthis. All treatments were administered via an intragastric tube. Diabetes was induced in the rats of groups 3 and 4 by an intraperitoneal injection with alloxan. Results: The liver of Citrullus colocynthis-treated rats revealed minor histological changes versus the control animals. In group 3 animals, diabetes caused degenerative alterations in the form of disorganization of the hepatic cords, cytoplasmic vacuolization and pyknosis of the nuclei of hepatocytes and inflammatory cell infiltration. Scanning electron microscope examination of these livers revealed numerous lipid droplets within hepatocytes, damaged blood sinusoids and hemorrhage of erythrocytes between hepatocytes and inside Disse's spaces. On the other hand, the normal histological and scanning ultrastructural features were nearly resumed in the liver of diabetic rats treated with Citrullus colocynthis pulp extract. Conclusion: The present study proved a lessening effect of Citrullus colocynthis pulp extract on the liver of diabetic rats. In light of these advantageous influences, it is advisable to widen the scale of its use in a trial to alleviate the diabetic hepatic adverse effects.

Copyright

© 2010 Mohammad Khalil, Gamal Mohamed, Mohammad Dallak, Fahaid Al-Hashem, Hussein Sakr, Refaat A. Eid, Mohamed A. Adly, Mahmoud Al-Khateeb, Saleh Banihani, Zuhair Hassan and Nabil Bashir. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.