American Journal of Biochemistry and Biotechnology

Effect of Nitrogen Source and Carbon to Nitrogen Ratio on Hydrogen Production using C. acetobutylicum

Mohd Sahaid Kalil, Hisham Salem Alshiyab and Wan Mohtar Wan Yusoff

DOI : 10.3844/ajbbsp.2008.393.401

American Journal of Biochemistry and Biotechnology

Volume 4, Issue 4

Pages 393-401

Abstract

Problem statement: One of the main factors influenced the bacterial productivity and total yield of hydrogen is the nitrogen source and its concentration. Approach: Using different nitrogen source with different concentration on bacterial productivity of hydrogen showed to affect on both bacterial productivity of hydrogen and biomass concentration. Results: Yeast extract as nitrogen source at concentration of 13 g L-1 was the best organic nitrogen source and resulted in hydrogen yield YP/S of 308 mL g-1 glucose utilized with biomass concentration of 1.1 g L-1, hydrogen yield per biomass YP/X of 280 mL g-1 L-1, biomass per substrate utilized YX/S of 0.22 and produced hydrogen in gram per gram of glucose utilized YH2/S of 0.0275. C/N of 70 enhanced the YP/S from 308-350 mL g-1 glucose utilized with biomass concentration of 1.22 gL-1, YP/X of 287 mL g-1 L-1, YX/S of 0.244 and YH2/S of 0.03125. Conclusion: Nitrogen source with proper C:N ratio enhanced the hydrogen production.

Copyright

© 2008 Mohd Sahaid Kalil, Hisham Salem Alshiyab and Wan Mohtar Wan Yusoff. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.