American Journal of Biochemistry and Biotechnology

New Bacterial Species Isolated from Malaysian Sea Cucumbers with Optimized Secreted Antibacterial Activity

Abd El-Aziem Farouk, Faizal Abd. Hamid Ghouse and B.H. Ridzwan

DOI : 10.3844/ajbbsp.2007.60.65

American Journal of Biochemistry and Biotechnology

Volume 3, Issue 2

Pages 60-65

Abstract

In this study, several Malaysian sea cucumber species that have traditional medicine value were selected and from them, the native bacterial population was isolated. Optimization of growth was designed and all bacterial secretions were tested for antibacterial properties. 30 bacterial types were isolated and 7 types recorded moderate antibacterial activity against K. pneumoniae, S. marscens, P. aeruginosa and E. feacalis. Antibacterial plate screening was done, with various testing parameters. Turbidometry revealed a single dose of the 10x concentrated crude antibacterial extracts were effective in preventing pathogenic growth for up to 4 hrs. PCR and subsequent sequencing of the 16S rDNA showed that the bacterial species were from the halopholic Bacillus and Klebsiella genera.

Copyright

© 2007 Abd El-Aziem Farouk, Faizal Abd. Hamid Ghouse and B.H. Ridzwan. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.