American Journal of Biochemistry and Biotechnology

Immobilization of Lipase from Candida Rugosa on Palm-Based Polyurethane Foam as a Support Material

Roila Awang, Mohd Rafaei Ghazuli and Mahiran Basri

DOI : 10.3844/ajbbsp.2007.163.166

American Journal of Biochemistry and Biotechnology

Volume 3, Issue 3

Pages 163-166

Abstract

Lipase from Candida rugosa was immobilized onto palm-based polyurethane foam (PU), which the polymer was pre-soaked in co-immobilized agent. The activities of PU-immobilized lipase were tested by the esterification reaction of oleic acid and oleyl alcohol in hexane. The PU-immobilized lipase was then characterized in term of its thermal, operational and storage stability. The optimum temperature for native and PU-immobilized lipase was at 40oC. This shows that the immobilization did not alter the general character of the lipase. The PU-immobilized lipase shows different enzymatic characteristic-incubation time, enzyme concentration, solvent and operational stability compared to Lipozyme IM. The reuse stability of PU-immobilized lipase was at least four cycles. The % conversion above 80% was still achieved for the sample stored at -5oC after 9 days storage.

Copyright

© 2007 Roila Awang, Mohd Rafaei Ghazuli and Mahiran Basri. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.