Role of Plasmid in Production of Acetobacter Xylinum Biofilms
Abbas Rezaee, Sanaz Solimani and Mehdi Forozandemogadam
DOI : 10.3844/ajbbsp.2005.121.124
American Journal of Biochemistry and Biotechnology
Volume 1, Issue 3
Acetobacter xylinum has the ability to produce cellulotic biofilms. Bacterial cellulose is expected to be used in many industrial or biomedical materials for its unique characteristics. A. xylinum contains a complex system of plasmid DNA molecules. A 44 kilobases (kb) plasmid was isolated in wild type of A. xylinum. To improve the cellulose producing ability of A. xylinum, role of the plasmid in production of cellulose was studied. The comparisons between wild type and cured cells of A. xylinum showed that there is considerably difference in cellulose production. In order to study the relationship between plasmid and the rate of cellulose production, bacteria were screened for plasmid profile by a modified method for preparation of plasmid. This method yields high levels of pure plasmid DNA that can be used for common molecular techniques, such as digestion and transformation, with high efficiency.
© 2005 Abbas Rezaee, Sanaz Solimani and Mehdi Forozandemogadam. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.