Occurrence of Feline Immunodeficiency Virus (FIV) and Feline Leukemia (FeLV) in São Luís-MA
Nathálya dos Santos Martins, Ana Paula Sousa Rodrigues, Stefanne Aparecida Gonçalves, Ana Lucia Abreu Silva, Rudson Almeida de Oliveira, Jenner Karlisson Pimenta dos Reis and Ferdinan Almeida Melo
DOI : 10.3844/ajavsp.2015.187.192
American Journal of Animal and Veterinary Sciences
Volume 10, Issue 3
The aim of this study was to evaluate the occurrence of feline immunodeficiency virus and feline leukemia virus in the city of SÃ£o LuÃs, MaranhÃ£o state (MA), Brazil. A total of 120 blood samples were tested using the Polymerase Chain Reaction (PCR) to detect the proviral DNA, with an amplification of a fragment of 244bp of the FIVâs gene gag. To detect anti-FIV antibodies and the p27antigen of FeLV, the samples were tested with a commercial immunoassay kit, SNAP Combo Plus (IDEXXÂ® Laboratories, USA), an indirect ELISA was also carried out for FIV. All three tests used gave 18.33% (22/120) of positivity for FIV. PCR and indirect ELISA found 10.83% (13/120) of the animals with FIV, while the SNAP Combo Plus found 9.17% (11/120). Only 0.83% (1/120) of the samples were positive for FeLV in the SNAP combo plus. Comparatively, SNAP combo plus showed higher sensitivity, whereas the indirect ELISA and PCR had, similar and better specificity (p<0.05). The small difference between the tests (all above 90%) indicates that they are highly specific and have a high VPN, implying that they have the capacity to distinguish true negatives. Therefore, this study concluded that FIV is present in SÃ£o LuÃs and that the clinical disease exists in MaranhÃ£o state, whereas for FeLV, there was only one seropositive animal which did not show any symptoms.
© 2015 Nathálya dos Santos Martins, Ana Paula Sousa Rodrigues, Stefanne Aparecida Gonçalves, Ana Lucia Abreu Silva, Rudson Almeida de Oliveira, Jenner Karlisson Pimenta dos Reis and Ferdinan Almeida Melo. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.