@article {10.3844/ajavsp.2015.187.192,
article_type = {journal},
title = {Occurrence of Feline Immunodeficiency Virus (FIV) and Feline Leukemia (FeLV) in São Luís-MA},
author = {Martins, Nathálya dos Santos and Sousa Rodrigues, Ana Paula and Gonçalves, Stefanne Aparecida and Abreu Silva, Ana Lucia and de Oliveira, Rudson Almeida and dos Reis, Jenner Karlisson Pimenta and Melo, Ferdinan Almeida},
volume = {10},
number = {3},
year = {2015},
month = {Jul},
pages = {187-192},
doi = {10.3844/ajavsp.2015.187.192},
url = {https://thescipub.com/abstract/ajavsp.2015.187.192},
abstract = {The aim of this study was to evaluate the occurrence of feline immunodeficiency virus and feline leukemia virus in the city of São Luí­s, Maranhão state (MA), Brazil. A total of 120 blood samples were tested using the Polymerase Chain Reaction (PCR) to detect the proviral DNA, with an amplification of a fragment of 244bp of the FIV's gene gag. To detect anti-FIV antibodies and the p27antigen of FeLV, the samples were tested with a commercial immunoassay kit, SNAP Combo Plus (IDEXX® Laboratories, USA), an indirect ELISA was also carried out for FIV. All three tests used gave 18.33% (22/120) of positivity for FIV. PCR and indirect ELISA found 10.83% (13/120) of the animals with FIV, while the SNAP Combo Plus found 9.17% (11/120). Only 0.83% (1/120) of the samples were positive for FeLV in the SNAP combo plus. Comparatively, SNAP combo plus showed higher sensitivity, whereas the indirect ELISA and PCR had, similar and better specificity (p},
journal = {American Journal of Animal and Veterinary Sciences},
publisher = {Science Publications}
}