Nutritional Value of the Maize Stalk Borer and American Bollworm as Unconventional Protein Sources

Problem statement: The maize stalk borer and American bollworm were grown in an artificial media to evaluate their potential as human food sources. Approach:  Both cultures were started from moths and the life cycle and culture structures were evaluated. Results: The larvae of both insects reached maximum weight and maximum length at the same time. The growth rate of the youngest larvae was found to be the highest and the increase in the body weight during the growth period appeared to be linear. The larvae of these insects started to decrease in weight after they reached the maximum size. For efficient systems for the commercial production of the maize stalk borer and the American bollworm, the larvae should be harvested when they are 39 and 21 days old, respectively. The protein contents were 38.0 and 33.0% and the fat contents were 14.5 and 14.4% for the dried larvae of the American bollworm and maize stalk borer, respectively. The larvae of both insects contained the essential amino acids and minerals. Only 10% mortality was observed with older larvae of both species. A system where eggs are separated from adults and hatched in separate chambers would alleviate the possible danger of losing the population due to microbial infection. The high moisture content of the larvae (60.4-61.0%) could cause handling and storage problems. Drying and grinding the larvae would reduce them to easily manageable forms and would improve their marketability as novel food. Conclusion: The results obtained from this study show the potential of using insects as a protein source for human consumption to alleviate protein deficiency in many parts of the world, especially in Latin America, Africa and Asia. Further research is required to evaluate their growth characteristics on low substrates. Future research should also evaluate environmental parameters such as temperature, relative humidity and heat and CO2 production on food consumption and protein yield per gram substrate. This information will aid in the design of optimal production systems.


INTRODUCTION
The world is coming to recognize the grim truth that ultimately the population growth will outstrip food suppliers with apocalyptic results. In 2000, 36 million people died due to hunger or as a result of hunger (FAO/SD, 2001). About 60% of the 10.9 million deaths each year among children under the age of five in the developing world are attributed to malnutrition (WHO, 2002). If the current average birth rate continues, the world's population will grow from the current 6.7-9.2 billion by the year 2050, most of which will be in the less developed countries of Latin America, Africa and Asia (WHO, 2007), the countries least able to feed their children. Feeding 9.2 billion people at the current dietary levels presents the staggering necessity of increasing the earth's food producing capacity to a rate never seen before.
Protein deficiency is one of the major nutritional problems in the developing world (Latham, 1997). Most foods rich in protein are also comparatively good sources of the other required nutrients (Eby and Dandy, 1962). If animal proteins supply part of the diet, the nutritional quality of the diet is generally enhanced because most animal proteins contain all the essential amino acids (cysteine, histidine, isoleucine, methionine, leucine, lysine, phenylalanine, methionine, tryptophan, tyrosine and valine) humans require, whereas most plant proteins lack one or more of these essential amino acids (NLM., 2007). Animal proteins are also a rich source of vitamin B 12 and minerals (Herbert, 1998). Because the body's need for protein is related to its rate of growth, the need for protein is much greater in infants than in older children or adults (Verkerk et al., 2007). There is agreement among scientists that protein malnutrition contributes to the high death rate among infants and children of the less developed countries and causes among the survivors debilitating weakness, higher susceptibility to disease and irreversible brain damage (Verkerk et al., 2007;Ramos-Elorduy, 1997).
The development of novel protein sources such as Fish Protein Concentrate (FPC) (Harrel et al., 1980;Pariser et al., 1977;Sikka et al., 1979), Single Cell Protein (SCP) (Tannenbaum, S.R. and D.K. Wang, 1975;Ferrianti and Fiechter, 1983) and Soybean Protein (SBP) (Mendez et al., 2002;Bhatia and Greer, 2008) have made significant contributions toward the alleviation of the world's protein deficiency (Kuijer et al., 1999). However, there is still an estimated one billion people suffering from protein deficiency and malnutrition (WHO/FAO., 2007). It is, therefore, necessary that similar success be obtained by utilizing what seems to be an inexhaustible supply of insects as a protein source for human consumption. Insects make up (on the average) about two-thirds of the food of our common land birds and two-fifths of the food of adult fresh water fish. Turkeys, hogs and other domestic animals are often fattened on insects (Flint and Metcalf, 1979). Therefore, insects can be reared on readily available low substrates to provide a sustainable and nutritional supply of protein for human consumption to alleviate the world protein deficiency and maintain the ever increasing human populations (Kok et al., 1991). Insects can be easily reared to efficiently convert low quality substrates to high quality proteins. Careful production, processing and marketing procedures would make insects as acceptable as FPC, SCP and SBP. This will require substantial innovation in food production and processing technologies (Verkerk et al., Pariser et al., 1977;DeFoliart, 1999).
Objectives: Although many reports have been made on eating the maize stalk borer and the American bollworm, very little information is available on the nutritional quality of these insects. These insects can be easily reared and handled in the laboratory to obtain information for the development of economically and technically feasible commercial production systems for their use as a source of protein for human consumption. The specific objectives of this study were: (a) to develop an artificial ration for production of viable cultures of the maize stalk borer (Busseola fusca) and the American bollworm (Heliothis armigera), (b) to establish viable and strong cultures of these insects, (c) to study the life cycle and culture structure of each insect, (d) to determine the growth rates of the larvae and (e) to evaluate the nutritional values of the larvae.

Maize Stalk Borer (Busseola fusca):
The maize stalk borer (Busseola fusca) belongs to the family of night flying moths. It is from the order Lepidoptera and the family Noctuidae. The insect is distributed worldwide and is found in all provinces of Canada. The maize stalk borer is almost a universal plant feeder attacking any plant large enough to shelter it and soft enough that it can bore into. Generally, it prefers field crops (such as corn, sorghum, sugar cane, cereals, tobacco) ragweed, grasses, flowers (such as dahlias, hollyhocks, hyer lilies and steers) and vegetables (such as rhubarb, mint, peppers, potato and tomato) where it tunnels in their stalks. Most of the damage caused to the corn plant is due to the direct result of the caterpillars making holes by burrowing in the pith of the stalks, causing the whole plant to wilt with tip leaves first turning yellow or brown and dying. Later in the season, the caterpillars get into the maize cobs allowing a secondary infection by fungi that cause further damage to the developing seeds (Ebenebe et al., 2000).
The life cycle of the maize stalk borer (Busseola fusca) includes four stages: Egg, larva, pupa and moth ( Fig. 1) (USDA., 1899). The caterpillars hibernate over winter inside the old stem bases of the last season's maize plants. They pupate in the spring and remain in the form of pupae for 10 days until they emerge as adult moths. The adult moths leave their empty pupal skins in the stalks. The moths are darkish brownish grey and mostly fly in the evenings and early morning. Over a period of a few weeks, the female moth lays up to 800 eggs in batches in the fold of the maize leaves near the top of the funnels. A batch of eggs may comprise of 30-150 eggs on one plant. They are about 1 mm in diameter. They hatch after 7-10 days.  (Tracy, 1907) (%)  ------------------------------------------------------ The young larvae are deep purple or black in color. The larvae tend to migrate up and down the plant and away to adjacent plants before they are half grown. They grow to about 25-30 mm in length and become darker in color. They feed on leaves, flowers and cobs and are known as top grub. They go to another plant to attack it by making small holes in the base of the stem and eat out the center killing the plant. They feed inside the stem for 6 weeks and then pupate near the root of the plant. The pupa is brown and about 25 mm long. In about 2 weeks, the adult moth comes out of the pupa and starts the second generation by laying more eggs on the maize plant. There may be 2 and 3 generations in a season before overwintering (Ebenebe et al., 2000;Bijimarkers, 1985).

American bollworm (Heliothis armigera):
The American bollworm (Heliothis armigera) belongs to the family of night flying moths. It is from the order Lepidoptera and the family Noctuidae. It is of worldwide distribution and is found in all provinces of Canada. It is the most important pest of agricultural crops causing extensive damage to cotton, maize, tobacco, tomato, sunflower, wheat, pigeon peas and cabbage. Damage is done when the temperature begins to rise in spring. The insects seldom attacks foliage but prefer more juicy plant materials such as tomato fruits, tobacco tips, cotton bolls and developing maize cobs. The insect is known by different names: on cotton and tomato it is known as the American bollworm, on maize it is known as the earworm and on tobacco it is known the a budworm (Dimietry, 2005).
The life cycle of the American bollworm (Heliothis armigera) includes four stages: Egg, larvae, pupa and moth ( Fig. 1) (Tracy, 1907). The larva is a stout caterpillar of greenish or brownish color. The body is marked with two lines of darker color running length-wise along the sides of the back. The full grown larva is about 40-50 mm long. The caterpillars feed with their head inside the boll or tomato fruit with the posterior part of the body outside. The worm is cannibalistic, only one larva is found in one fruit. Pupation occurs in 7 days and the length of the pupation stage is influenced by temperature. The adult is a brown, nocturnal moth with a wing span of about 40 mm. The moths rest during the day with the folded wings on tree trunks and fly out to lay eggs on the plants at night. In about 10-20 days, several hundred (1000) eggs are laid singly. The eggs hatch in 2-4 days. The larval stage lasts for 15-20 days (Dimietry, 2005;Robinson, 1987).

Growth medium:
The proper consistency of the diet and provisions for an adequately balanced diet that contains the essential growth substances is necessary for the propagation of insects (Lipke, 1957;Gupta et al., 2004). A nutritionally complete diet for most insects must contain: (a) protein or essential amino acids, (b) carbohydrates, (c) fat or fatty acids (cholesterol), (d) vitamins (ascorbic acid, a-tocopherol, folic acid, inositol, nicotinamide, pantothenic acid, riboflavin, thiamine or vitamin A), (e) minerals and (f) water. In this study, two artificial diets were prepared (Table). The first was used for rearing young insect larvae for the propagation of starter culture and the determination of weight and length and the second was used for mass rearing of insects for the determination of nutritional value.
Starter diet: This diet was prepared according to the procedure described by Guptal et al. (2004);Haydaks (1936) with slight modification. The diet was prepared by mixing corn flour, whole wheat flour, wheat bran and dried yeast powder (3:3:3:1 ratio by weight). The dry mixture was combined with an equal part of fluid made of glycerine and honey (1:1 ratio by weight). The mixture was mixed thoroughly and then allowed to stand for 24 h before use. The diet was then placed in oviposition and egg hatching cages.

Mass rearing diet:
The diet used for grown up caterpillars was made of corn flour, wheat bran, wheat germ, brewer's yeast, agar, milk, castor oil, chicken mash and water. The corn flour, wheat germ, wheat bran, brewer's yeast and agar (3:3:3:1:1 ration by weight) were mixed together, combined with an equal part of chicken mash and blended for 2 min. The mixture was combined with an equal part of fluid made of milk, castor oil and water (1:1:4 ration by weight), mixed thoroughly and cooked for 15 min. The diet was then poured into the mass rearing cages and allowed to stand for 24 h to solidify before use.
Starter culture: The culture of the maize stalk borer was started from adults emerging from old corn stalks whereas the culture of the American bollworm was started from adults emerging from old tomato plant material. Both infected materials were collected from infected fields in Truro, Nova Scotia.
Rearing moths: Rearing moths was done in an environmentally controlled chamber (VWR Environmental Chamber, Model No. 2020, Shelden Manufacturing Company Inc., Cornelius, Oregon) at a temperature of 28°C, a relative humidity of 70% and a photoperiodic regime of 10 h of light and 14 h of darkness. Adult moths from each species were used for egg-laying. The starter medium was placed in the oviposition cages (60 cm long × 40 cm wide × 25 cm deep plastic container with perforated sides and cover) and arranged to provide the greatest surface area. Ten seeding adults from each insect species were placed over the medium in separate oviposition cages and used to produce the next generation. Waxed paper sheets were provided for the moths to lay their eggs.

Hatching of eggs:
The starter medium was placed in each of the egg hatching chambers (40 cm long × 40 cm wide × 25 cm deep plastic container with perforated sides and cover). The waxed papers (with eggs) were collected from the oviposition cages and the number of eggs per waxed paper sheet was estimated for each insect species. 100 eggs from each container were placed in several petri dishes and left on the top of the medium. The egg hatching chambers were placed in an environmentally controlled chamber (VWR Environmental Chamber, Model No. 2020, Shelden Manufacturing Company Inc., Cornelius, Oregon) at 28°C, 70% relative humidity and a photoperiodic regime of 10 h light and 14 h of darkness. Emerging larvae were isolated and 10 larvae were selected for the study of growth rate and life cycle while the rest of the larvae were mass reared using the mass rearing medium for the determination of nutritional value.
Growth rate determination: Ten larvae from each species were used for the length and weight measurements and growth rate determination. Each group of larvae were placed in separate rearing cages (60 cm long × 45 cm × wide × 25 cm × deep plastic containers with perforated plastic sides and cover) and reared on the prepared starter diet. The containers were placed in the growth chamber (VWR Environmental Chamber, Model No. 2020, Shelden Manufacturing Company Inc., Cornelius, Oregon) at a temperature of 28°C, a relative humidity of 70% and a photoperiodic regime of 10 h day light and 14 h of darkness. The larvae of each species were weighed as a group every 3 days. The average length of the larvae was also measured and recorded. The changes in the groups were observed and recorded.
Mass rearing: The rest of the larvae of each species were taken from the starter culture and used as stock for mass rearing of the insects for determination of nutritional value. They were all of similar age and reared to maturity. The mass rearing medium was placed in the mass rearing cages (65×45×45 cm deep plastic container with perforated sides and cover). The mass rearing cages were placed in an environmentally controlled chamber (VWR Environmental Chamber, Model No. 2020, Shelden Manufacturing Company Inc., Cornelius, Oregon) at a temperature of 28°C, relative humidity of 70% and photoperiodic regime of 10 h of light and 14 h of darkness. Two, one litre jars were provided for the larvae to pupate inside. The pupae were collected from the bottles and placed in special containers filled with medium until the emergence of the moths. Adults were allowed to emerge into the jars where they were paired for appropriate mating. The female moths were collected and placed in the oviposition cases.

Chemical analyses:
The chemical analyses performed in this study included, moisture, ash, protein and fat contents and mineral and amino acid profiles.
Moisture content: A sample of thirty mass reared larvae from each species representing a full range of weighs were chosen the moisture analysis. The oven dry method described by APHA (1990) was followed. The worms were frozen and dried in a freeze dryer (Labconco FreeZone, Cat No. 10-271-16, Fisher Scientific, Montreal, Quebec) for 24 h. The weight of each group was recorded using a Mettler scientific balance (AE 2005, Mettler Instruments, AG, Greifensee, Zurich, Switzerland). They were then placed in a convection oven (Isotempoven, Model No. 655F, Fisher Scientific, Montreal, Quebec) for 24 h at 105°C. The dried samples were then removed from the oven, left to cool in a dessicator and weighed. The moisture content was calculated as follows: Where: MC = The moisture content (%) M 1 = The initial weight (g) M 2 = The weight of the dried sample (g) Ash content: The ash content was determined gravimetrically on the dried worm samples according to the procedure described in APHA (1990 Where: AC = The ash content (%) M 3 = The weight of the material remaining after burning the dry sample (g) Where: PC = The protein content (%) W s = The weight of the freeze dried worms (g) Fat content: The fat content was carried out, using 30 mass reared larvae from each species representing a full range of weights. The worms were frozen and dried in a freeze dryer (Labconco FreeZone,Fisher Scientific,Montreal,Quebec) for 24 h. The weight of each group (30 worms) was recorded using a Mettler scientific balance (AE 2005, Mettler Instruments, AG, Greifensee, Zurich, Switzerland). Each group was ground in a laboratory grinder (Cat No. 14-509-18, Waring Laboratory, Fisher Scientific, Montreal Quebec). The fat content was determined using an ether extraction technique according to the procedure described in the Official Method of the Association of Official Analytical Chemists (AOAC) (1975). Hot ether was percolated through a porous receptacle filled with the dry ground meal worms for 24 h. The fat was released from the dry matter and collected in a flask at the bottom of the apparatus.

Life cycle and culture structure:
The stages of the life cycle and the changes among the individuals in each group of insects were observed and recorded. These included the number of eggs per waxed paper, the total number of eggs, the average weight of larvae, the average length of larvae, the average lengths of oviposition, pupal and adult stages, the mortality among the larvae and the appearance. The changes were of three types: (a) Death of one or more of the worms, (b) pupation of one or more of the worms and (c) the pupation of one or more of the worms. The dead worms were counted and discarded. The number of worms to enter the pupal stage of their development and the number of adults emerging from the pupal stage were recorded for each group. The results are shown in Table 2 and 3. The population structure is shown in Fig. 2. Only one larva died (10%) from each group of insects. The phenomenon of cannibalism among the American bollworm reported by Chipeleme (Chipeleme, 1981) was not observed in this study. The pupation stage started at different times for the two species. The pupation started on days 33 and 12 and emergence of adults started on days 51 and 21 for the maize stalk borer and American bollworm, respectively. The pupation stage lasted for 21 and 15 days and the adult stage lasted for 12 and 15 days for the maize stalk borer and American bollworm, respectively. The maximum length was 30 and 50 mm and the maximum weight was 2.50 and 5.30 g for the maize stalk borer and American bollworm, respectively.
Growth rate: For each species of insects, the average weight and average length were plotted against time as shown in Fig. 3. It was noticed that for the first 3-6 days, the average weight did not show any significant increase. This initial period was required for the adjustment to the new environment and medium. The weight of larva increased with time up to a maximum value and then decreased once the larva was preparing for the pupation stage. The maximum weight and maximum length were reached at the same time.  Length (   The change in growth rate (g day −1 ) was determined by dividing the weight increase in a given period by the length of the period (3 day). The results are shown in Fig. 4. Initially, the growth rate increased and then decreased when the larvae got older. The negative values indicated weight loss.

Growth and time indices:
The first 3-6 days were considered a period of adjustment to the new environment (slow or no growth) and the period of pupation was associated with weight loss. Thus, the period of larval growth (continued growth) was linearized as shown in Fig. 5. The slope of the linear line (average growth rate) was divided by the initial weight to obtain the growth index. The time required to reach the maximum weight was divided by the maximum weight of the larva to obtain the larval time index. The time required to reach the maximum weight was also divided by the total population weight (number of eggs produced by female moth × larval weight) to obtain the population time index. The results are shown in Table 4. The average rate of growth achieved was 0.09 and 0.39 g day −1 for the maize stalk borer and the American bollworm, respectively. The time required to reach maximum size was 39 and 21 days for the maize stalk borer and American  bollworm, respectively. The time required to produce one gram of larva was 15.60 and 3.96 days for the maize stalk borer and American bollworm, respectively. When the population size (number or eggs produced by female moth) was considered, the time required to produce one gram of insects was 0.47 and 0.10 for the maize stalk borer and American bollworm, respectively.

Larval composition:
The results of moisture, ash, carbohydrate, protein and fat contents were calculated on dry weight basis when the larvae reached their maximum weights. The results are presented in Table 5. Although the larvae of both insect species were dried under the same conditions, the maize stalk borer had slightly higher moisture content (12.2%) than the American bollworm (11.6%). The maize stalk borer also had higher ash, carbohydrate and fat contents (11.7, 22.6 and 16.7%, respectively) than the American bollworm (7.5, 20.1 and 16.1%, respectively). The American bollworm had higher protein content (44.7%) than the maize stalk borer (36.8%).
The energy available in the carbohydrate, protein and fat in the larvae are shown in Table 6. About 383 and 402 kcal/100g larvae are provided by the carbohydrate, protein and fat contents of the maize stalk borer and the American bollworm, respectively. The female moths of the maize stalk borer produced 800 eggs while the female moth of the American bollworm produced 1000 eggs. Therefore, about 7660 and 21306 kcal will be available in the larvae produced by the female moth of the maize stalk borer and American bollworm, respectively.
The quality of the protein and, thus, the nutritional value of the insects is determined by the amino acid composition as reported by DeGuevara et al. (1995). The larvae of both worms contain all the essential amino acids needed for human growth as shown in Table 7. Table 8 shows the profile of some of the essential elements found in the larvae of the maize stalk borer and American bollworm. The total elemental composition was 121.2 and 72.29 mg g −1 dry matter for the maize stalk borer and American bollworm, respectively. The larvae of both worms contain most of the essential elements. However, the maize stalk borer has higher concentrations of all the elements compared to the American bollworm.

DISCUSSION
The results showed an initial period of adjustment by the young larvae to the new environment. This was followed by a period of increased weight. The increase in the body weight during the growth period appeared to be linear. After the initial growth period, the larvae started to decrease in weight after they reached their maximum size. An interesting characteristic of the caterpillars studied is that they reached maximum weight and maximum length at the same time. For the commercial production of the maize stalk borer and the American bollworm, the larvae should be harvested when they are 39 and 21 days old, respectively.
Only 10% mortality was observed with older larvae of both species. A system where eggs are separated from adults and hatched in separate chambers would alleviate the potential danger of losing the population due to microbial infection. The high moisture content of the live larvae (61.0% for the maize stalk borer and 60.4 for the American bull worm) could cause handling and storage problems. Drying and grinding the larvae would reduce them to easily manageable forms that would improve their marketability as novel food.
Because the larvae seem to be a promising source of protein for human consumption, further research is required to evaluate their growth characteristics on low substrates. Future research should also evaluate environmental parameters such as temperature, relative humidity and heat and CO 2 production on food consumption and protein yield per gram substrate. This information will aid in the design of an optimal insect production system. The results obtained from this study show the potential of using insects as a protein source for human consumption to alleviate protein deficiency   Protein energy: 4 kcal g −1 ; Fat energy: 9 kcal g −1 ; Live moisture content = 61% for MSB and 60.4% for ABW; Dried moisture content = 11.3% for MSB and 10.6% for ABW; Live weight of larva = 2.5 g for MSB and 5.3 g for ABW; Number of eggs produced by female moth = 800 for MSB and 1000 for ABW Table 7: Amino acid profile g/100 g dry matter Amino acid     Table 9: Taste of commonly used insects (Holt, 1969;Taylor, 1975) Insect Taste  Taste like lobsters or snail with a faint smell of mushrooms in many parts of the world, especially in Latin America, Africa and Asia. Governments in these countries should embark on using these inexhaustible nutritional resources to feed their people. The species that have been proven to be high in protein and nutritional content and show the economics of energy conversion of feed to insect protein are good candidates for mass rearing and marketing Mass rearing of insects has been practiced for years. Bees are grown in large quantities for distribution as colonies to honey producers (Kok et al., 1991). Booram (1975) Boll weevils have been mass reared for pest control (Miller et al., 1975;Webb et al., 1981). Mealworms have been produced on a commercial scale as food for birds and reptiles (Ebeling, 1975). Some efforts have been made to produce flies from livestock waste for animal consumption (Gantt and King, 1981;Morgan and Eby, 1975;Burchell, 1989). It is, therefore, conceivable that insect farming will be a part of a new agricultural system. It will be possible in the near future to construct small but efficient insect farms that have high volumetric protein production rates. Insects are in size between SCP and farm animals, they yield structured animal protein, require much less energy for processing than SCP and less space than farm animals, still large enough to be reared and harvested using automated systems. They contain proteins, essential amino acids, lipids, minerals, vitamins and energy (Bukkens 1997;DeGuevara et al., 1995;DeFoliart and Professor, 1992).
In the light of current protein deficiency and population growth, there is a need for a novel protein source (as an alternative for meat) that is safe, nutritious, flexible, reliable and consumer accepted. It has been predicted that in the next decades about 40% of red bovine meat consumption will be replaced by novel protein sources such as insects (Verkerk et al., 2007). Although of small size, insects because of their prodigious numbers, exceed in weight all other animal matter (Banjo et al., 2006). This great mass of material possesses genuine food value. From the eating habits of wild animals by natives in many countries, it seems that much of the insects are palatable (Table 9) (Holt, 1969;Harris and Ross, 1987). Chemical analyses of many insects have also shown that they compare favourably with traditional foods/animal products (Table 10) (Ramos-Elorduy, 1997;Bukkens, 1997;Taylor, 1975).
Since a wide variety of low substrates are available, materials which are not edible can be converted into human food by insects such as mealworms. Insects are very efficient feed converters to protein (more than five times that of beef) and fast growing organisms (Banjo et al., 2006), two characteristics that allow the design of a farm system that can respond to changes in demand. The integration of insects into the current agricultural production system will increase the complexity of the systems by creating symbiotic relationships with other species. This will improve the efficiency of the agricultural production system and insure its sustainability.

CONCLUSION
The larvae of both the maize stalk borer and American bollworm reached maximum weight and maximum length at the same time. The increase in the body weight during the growth period appeared to be linear. The larvae started to decrease in weight after they reached their maximum size. For the commercial production of the maize stalk borer and the American bollworm, the larvae should be harvested when they are 39 and 21 days old, respectively.
Only 10% mortality was observed with older larvae of both species. A system where eggs are separated from adults and hatched in separate chambers would alleviate the danger of losing the population due to microbial infection. Drying and grinding the larvae would reduce them to easily manageable forms and would improve their marketability as novel food.
The results obtained from this study show the potential of using insects as a protein source for human consumption to alleviate protein deficiency in many parts of the world, especially in Latin America, Africa and Asia. Governments in these countries should embark on using these inexhaustible nutritional resources to feed their people. The species that have been proven to be high in protein and nutritional contents and show the economics of energy conversation of feed to insect protein are good candidates for mass rearing.
Because the larvae seem to be a promising source of protein for human consumption, further research is required to evaluate their growth characteristics on low substrates.
Future research should evaluate environmental parameters such as temperature, relative humidity and heat and CO 2 production on food consumption and protein yield per gram substrate. This information will aid in the design of an economically sustainable insect production system.