Research Article Open Access

Analysis of Salmonella enteritidis Outer Membrane Proteins and Lipopolysaccharide Profiles with the Detection of Immune Dominant Proteins

A. Maripandi1 and Ali A. Al-Salamah2
  • 1 KS Rangasamy College of Arts and Science, India
  • 2 King Saud University, Saudi Arabia

Abstract

Problem statement: Salmonella infection is a serious medical and veterinary problem world-wide and causes great concern in the human health and food safety. Salmonella enteritidis outer membrane proteins and lipopolysaccharide play important role in the virulence and immunological properties of bacteria. Prophylaxis of enterobacterial infection has proven difficult to achieve. Vaccination is an effective tool for the prevention of Salmonella infection. Approach: Therefore, a study was conducted on S. enteritidis isolates from chicken meat samples for Outer Membrane Proteins (OMPs) and Lipopolysaccharide (LPS) profile were analyzed by SDS-PAGE electrophoresis and immunodominent antigen was detected by western blot analysis. Results: S. enteritidis with different OMPs bands were exhibited with molecular weight ranging from 5-90 kDa. The major outer membrane protein profiles of all S. enteritidis isolates were homogenous with different expression in intensity of protein was observed. LPS of different S. enteritidis isolates exhibited doublet band was observed and identified as R type of strains. The immunoblotting results at 14.4 and 24 KDa proteins were good immunogen. Conclusion: The 14.4 and 24 KDa proteins were immune response protein. This proteins can use for vaccine development.

American Journal of Immunology
Volume 6 No. 1, 2010, 1-6

DOI: https://doi.org/10.3844/ajisp.2010.1.6

Submitted On: 4 May 2010 Published On: 21 June 2010

How to Cite: Maripandi, A. & Al-Salamah, A. A. (2010). Analysis of Salmonella enteritidis Outer Membrane Proteins and Lipopolysaccharide Profiles with the Detection of Immune Dominant Proteins. American Journal of Immunology, 6(1), 1-6. https://doi.org/10.3844/ajisp.2010.1.6

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Keywords

  • Salmonella enteritidis
  • outer membrane protein
  • immune-dominant antigen