@article {10.3844/ojbsci.2025.742.749, article_type = {journal}, title = {Larvicidal Activity of Spondias purpurea (Sineguelas) Leaf and Hylocereus costaricensis (Dragon Fruit) Stem Extracts Against Aedes aegypti}, author = {Corpuz, Alfredo V. and Gallardo, Nigell Mikhail A. and Avero, Ace Danielle C.}, volume = {25}, number = {3}, year = {2025}, month = {Nov}, pages = {742-749}, doi = {10.3844/ojbsci.2025.742.749}, url = {https://thescipub.com/abstract/ojbsci.2025.742.749}, abstract = {The study determined the larvicidal activity of the different concentrations of Spondias purpurea and Hylocereus costaricensis extracts by measuring percent mortality in Aedes aegypti larvae, a primary vector of dengue and other arboviral diseases. The hypothesis proposed that combining both extracts would yield greater larvicidal activity due to potential synergistic interactions. Ethanolic extraction was used to isolate a wide array of phytochemicals. Third- to fourth-instar larvae were exposed to the extracts using WHO standard bioassay methods, and mortality was recorded over a six-hour period. Phytochemical screening revealed active compounds such as alkaloids, saponins, flavonoids, tannins, phenolics, and glycosides. Results showed that the 100% concentrations of both individual extracts had high larvicidal activity, while their 100:100% combination exhibited mortality comparable to 1% Malathion, the positive control. Statistical analysis confirmed significant differences between treatments and control groups (p}, journal = {OnLine Journal of Biological Sciences}, publisher = {Science Publications} }