@article {10.3844/ojbsci.2025.408.416,
article_type = {journal},
title = {Nerve Growth Factor (NGF) Gene Analysis in Menstrual Blood of Endometriosis: DNA Methylation and mRNA Expression Levels},
author = {, Ocktariyana and Hikmawati, Nurul and Muharam, Raden and Hestiantoro, Andon and Marwali, Muhammad Luky Satria Syahbana and Surur As’adi, Agus and  , Asmarinah},
volume = {25},
number = {2},
year = {2025},
month = {Aug},
pages = {408-416},
doi = {10.3844/ojbsci.2025.408.416},
url = {https://thescipub.com/abstract/ojbsci.2025.408.416},
abstract = {Menstrual blood flow is reversed through the fallopian tubes during the menstrual process and implants outside the uterus, which is the theory of pathogenesis in endometriosis sufferers. This study aims to analyze the level of DNA methylation and mRNA expression of the Nerve Growth Factor (NGF) gene in menstrual blood in endometriosis patients and analyze the correlation. The study was conducted among 20 women in each group with and without Endometriosis (controls) of reproductive age. Filter paper pads were used to collect the menstrual blood sample, while the eutopic endometrium was collected by biopsy. Following that, NGF DNA methylation analysis of menstrual blood was performed using Methylation-Specific PCR (MS-PCR) and Pyrosequencing. Additionally, the study of NGF mRNA expression was conducted by RT-qPCR. Data analysis using independent T-test or Mann-Whitney, SPSS 22, and p},
journal = {OnLine Journal of Biological Sciences},
publisher = {Science Publications}
}