TY  - JOUR
AU  - Filimonova, Maria 
AU  - Gubskaya, Valentina 
AU  - Nuretdinov, Il’dus 
PY  - 2014
TI  - SOME FEATURES OF HYDROLYSIS OF THE HYBRID B-Z-FORM DNA BY SERRATIA MARCESCENS NUCLEASE
JF  - OnLine Journal of Biological Sciences
VL  - 14
IS  - 3
DO  - 10.3844/ojbsci.2014.181.187
UR  - https://thescipub.com/abstract/ojbsci.2014.181.187
AB  - Highly polymerized herring testis DNA of the random nucleotide sequence was used as a model of natural substrate to study some features of hydrolysis of the hybrid B-Z form with Serratia marcescens nuclease. The hybrid B-Z-form was formed upon addition of 1.15 M MgSO4 and 0.421 mM Co(NH3)6Cl3. The DNA transition from the right handed B-form to the hybrid B-Z-form caused a decrease in Vmax of DNA cleavage with the nuclease. The diminishing Vmax was consistent with diminishing values of Km and Kcat. The binding of Mg2+ or Co(NH3)63+ to highly polymerized DNA caused correspondingly about 80-or 7-fold decrease in Km and more than 1600 or 600 decrease in Kcat compared with that of Mg-DNA complex of B-form.