@article {10.3844/ojbsci.2014.181.187,
article_type = {journal},
title = {SOME FEATURES OF HYDROLYSIS OF THE HYBRID B-Z-FORM DNA BY SERRATIA MARCESCENS NUCLEASE},
author = {Filimonova, Maria and Gubskaya, Valentina and Nuretdinov, Il’dus},
volume = {14},
number = {3},
year = {2014},
month = {Jul},
pages = {181-187},
doi = {10.3844/ojbsci.2014.181.187},
url = {https://thescipub.com/abstract/ojbsci.2014.181.187},
abstract = {Highly polymerized herring testis DNA of the random nucleotide sequence was used as a model of natural substrate to study some features of hydrolysis of the hybrid B-Z form with Serratia marcescens nuclease. The hybrid B-Z-form was formed upon addition of 1.15 M MgSO4 and 0.421 mM Co(NH3)6Cl3. The DNA transition from the right handed B-form to the hybrid B-Z-form caused a decrease in Vmax of DNA cleavage with the nuclease. The diminishing Vmax was consistent with diminishing values of Km and Kcat. The binding of Mg2+ or Co(NH3)63+ to highly polymerized DNA caused correspondingly about 80-or 7-fold decrease in Km and more than 1600 or 600 decrease in Kcat compared with that of Mg-DNA complex of B-form.},
journal = {OnLine Journal of Biological Sciences},
publisher = {Science Publications}
}