TY - JOUR AU - Hassina, Guetarni AU - Ahmed, Bensoltane PY - 2013 TI - ISOLATION AND CHARACTERIZATION OF HELICOBACTER PYLORI STRAINS FROM GASTRIC BIOPSIES OF ALGERIAN PATIENTS JF - OnLine Journal of Biological Sciences VL - 13 IS - 2 DO - 10.3844/ojbsci.2013.41.49 UR - https://thescipub.com/abstract/ojbsci.2013.41.49 AB - The objective of this study is to isolate and characterize strains Helicobacetr pylori from gastric biopsies by upper gastrointestinal endoscopy. After a rapid urease test, two Helicobacter pylori strains isolated from four patients on blood agar. After extraction of DNA from these strains from homogenates of gastric biopsies, quantification by real-time PCR was performed to determine Helicobacter pylori on the one hand and sensitivity to clarithromycin species. Melting curve analyzes showed that these two strains are sensitive to the antibiotic.