@article {10.3844/ajptsp.2010.133.138,
article_type = {journal},
title = {Characterization of Epoxidized and Non-Epoxidized Fatty Diethanolamides by High Performance Liquid Chromatography and Gas Chromatography},
author = {Lee, Choy Sin and Lee, Song Cang},
volume = {5},
year = {2010},
month = {Sep},
pages = {133-138},
doi = {10.3844/ajptsp.2010.133.138},
url = {https://thescipub.com/abstract/ajptsp.2010.133.138},
abstract = {Problem statement: This study was carried out for the characterization of epoxidized and
non-epoxidized fatty diethanolamides by High Performance Liquid Chromatography (HPLC) and Gas
Chromatography (GC). Approach: The fatty diethanolamides were synthesized by reacting 40%
(w/w) of Epoxidized Palm Olein (EPOo) with 60% (w/w) Refined Bleached Deodorized Plam Kernel
Olein (RBDPKOo) with Diethanolamine (DEA) at 110°C for 5 h. Results: The identities of the peaks
were established by secondary standards that were derived from pure methyl esters. HPLC separated
major non-epoxidized diethanolamide compounds, namely C10:0, C12:0, C14:0, C16:O, C18:1 and C18:0 and
the molecular weights of the compounds were dentified by Liquid Chromatography-Mass
Spectrometry (LC-MS). Conclusion: GC provided a more complete separation for the non-epoxidized
diethanolamides, epoxidized diethanolamides, glycerol, Diethanolamine (DEA) and some minor
compounds from the self-condensation of DEA and diethanolamides. Gas Chromatography-Mass
Spectrometry (GC-MS) elucidated the chemical structure of the epoxidized diethanolamides.},
journal = {American Journal of Pharmacology and Toxicology},
publisher = {Science Publications}
}