@article {10.3844/ajisp.2009.101.107,
article_type = {journal},
title = {Foxp3-Mediated Immunity of Human Pancreatic Cancer Cell Line PANC-1},
author = {Wang, Renxi and Han, Gencheng and Wang, Jianan and Chen, Guojiang and Xu, Ruonan and Wang, Liyan and Li, Xia and Shen, Beifen and Li, Yan},
volume = {5},
year = {2010},
month = {Jan},
pages = {101-107},
doi = {10.3844/ajisp.2009.101.107},
url = {https://thescipub.com/abstract/ajisp.2009.101.107},
abstract = {Problem statement: More and more evidences have shown that human cancer cells can express Foxp3, the regulatory T cell-associated transcription factor. The role of Foxp3 in human cancer cells is still unclear. We detected Foxp3 expression in human pancreatic cancer cell line PANC-1. SiRNA assays showed that Foxp3 expression suppressed the expression of TGF-β and IL-10 which could induce immune tolerance. Approach: Thus, we proposed that Foxp3 expression in PANC-1 cells controlled the response but not tolerance characteristics of the cells. To prove the proposal, we first developed the PANC-1 cell-induced the immune response model. PANC-1 cells induced response in autoimmune Non-Obese Diabetes (NOD) mice aged of >12 weeks. In vitro cell co-cultured assay demonstrated that PANC-1 induced response in splenocytes from NOD mice aged of >12 weeks. Results: In the same time, we found that inflammatory cytokine such as INF-γ, IL-2 and IL-17 increased and anti-inflammatory cytokine such as IL-4, IL-10 and TGF-β decreased in the co-cultured supernatant. These results demonstrate that PANC-1 cells induced response in splenocytes from NOD mice aged of >12 weeks. SiRNA assays showed that Foxp3 in PANC-1 cells controlled the response in the co-cultured cells. Conclusion: The results suggested that Foxp3 control the immunological response characteristics of PANC-1 cells in autoimmune condition.},
journal = {American Journal of Immunology},
publisher = {Science Publications}
}