TY - JOUR AU - Wang, Ce AU - Zhao, Ouya AU - Sun, Shiyou AU - Ru, Shuhua AU - Liu, Lei AU - Xiao, Guangmin AU - Wang, Xueqing AU - Hou, Limin AU - Gao, Jing AU - Li, Pin AU - Sun, Shiqin AU - Wang, Ling PY - 2025 TI - The Effect of Different Functional Microbial Agents on the Biological Characteristics and Rhizosphere Enzyme Activity of Hydroponic Cucumber JF - American Journal of Biochemistry and Biotechnology VL - 21 IS - 1 DO - 10.3844/ajbbsp.2025.68.75 UR - https://thescipub.com/abstract/ajbbsp.2025.68.75 AB - This study aims to comprehensively explore the effect of functional microbial agents on healthy soil cultivation and attempt to increase cucumber yield. Three different microbial agents were used for cucumber seedlings by virtue of the hydroponic cultivation method in order to study their effects on enzyme activity and seedling growth and reveal the relationship between rhizosphere enzyme activity and carbon, nitrogen, and phosphorus cycling processes. The results showed that microbial agent 1 (BaCillus Mucilaginosus and Bacillus megaterium Preparations) significantly enhanced the enzyme activity in hydroponic cucumber solution and promoted the growth of cucumber plants. In addition, microbial agent 1 increased neutral protease activity by 20%, peroxidase activity by 49.7%, urease activity by 18.03% and cellulose activity by 3.42% in the rhizosphere solution of hydroponic cucumber. Overall, the comprehensive analysis of cucumber growth and enzyme activity in the rhizosphere solution has proved the best effect of a microbial agent on productive cucumber cultivation. This study suggests the promising perspective of functional microbial agents for crop high-yield production in the near future.