@article {10.3844/ajbbsp.2024.112.125,
article_type = {journal},
title = {Protective Role of Ganoderma lucidum Polysaccharides Against Stress Induced by Heavy Metals in Caenorhabditis elegans},
author = {Ma, Huixiu and Dai, Dehui and Chen, Guicai and Hu, Weilian},
volume = {20},
number = {2},
year = {2024},
month = {May},
pages = {112-125},
doi = {10.3844/ajbbsp.2024.112.125},
url = {https://thescipub.com/abstract/ajbbsp.2024.112.125},
abstract = {This study aimed to investigate the protective effects and mechanisms of different Ganoderma lucidum Polysaccharides (GLPs) on the damage caused by heavy metal ions. The polysaccharides (GLP-B, GLP-F, and GLP-S) were obtained from G. lucidum fruiting body powder, fermentation powder, and spore powder. The contents of polysaccharides in these sources were 0.95, 4.69, and 1.46%, respectively. The monosaccharide composition and functional groups of polysaccharides from different sources were analyzed by PMP pre-column derivatization and Fourier transform infrared spectroscopy, respectively. Results showed that the monosaccharide composition, molar ratio, and functional groups of them were significantly different. The antioxidant activities in vitro were investigated based on iron ion chelating ability and scavenging hydroxyl radical, superoxide radical, and DPPH free radical assays. Results showed that GLP-F, GLP-B, and GLP-S demonstrated significant antioxidant activity. Among them, the strongest effect of GLP-F for hydroxylic group radicals; GLP-S showed the strongest antioxidant capacity in superoxide radical scavenging; GLP-B showed the strongest antioxidant capacity in iron ion chelating. The four heavy metal ions stress model (Cu2+, Cd2+, Mn2+, and Ni2+) was established using the Caenorhabditis elegans. Results showed that the lifespan, locomotion behavior, and antioxidant index of C. elegans were significantly changed by heavy metal ions (p},
journal = {American Journal of Biochemistry and Biotechnology},
publisher = {Science Publications}
}