TY  - JOUR
AU  - Natalia, Dessy 
AU  - Heryakusuma, Christian 
AU  - Puspasari, Fernita 
AU  - Juniar, Linda 
AU  - Sugiyono, Yovin 
AU  - Yuwita, Anita 
AU  - Djauhari, Hofiya 
AU  - Riswari, Silvita F. 
AU  - Alisjahbana, Bachti 
AU  - Ihsanawati, 
PY  - 2018
TI  - Production of Immunologically Active Untagged Recombinant DENV-2 NS1 in Escherichia coli
JF  - American Journal of Biochemistry and Biotechnology
VL  - 14
IS  - 3
DO  - 10.3844/ajbbsp.2018.230.237
UR  - https://thescipub.com/abstract/ajbbsp.2018.230.237
AB  - Dengue is an infectious disease affecting 390 millions people in tropical and subtropical region annually. Dengue virus NS1 protein plays an important role in viral replication in the host cell and it is detected in high level in the infected patient serum. A synthetic gene of untagged DENV-2 NS1 with codons optimization for expression in Escherichia coli has been generated and then inserted into an expression vector pET16b. As a hydrophobic protein with aliphatic index of 71.21%, DENV-2 NS1 was produced as inclusion bodies in E. coli BL21(DE3). The DENV-2 NS1 aggregate was unfolded in 8 M urea and then refolded by reverse dilution method. The refolded DENV-2 NS1 has immunologically active structure as it is capable to interact with anti-NS1 antibody, hence making it as a potential vaccine candidate.