@article {10.3844/ajbbsp.2014.81.87,
article_type = {journal},
title = {DEVELOPMENT OF A BACTERIAL CULTURE SYSTEM USING A PAPER PLATFORM TO ACCOMMODATE MEDIA AND AN INK-JET PRINTING TO DISPENSE BACTERIA},
author = {Srimongkon, Tithimanan and Ishida, Takuya and Igarashi, Kiyohiko and Enomae, Toshiharu},
volume = {10},
number = {1},
year = {2014},
month = {Apr},
pages = {81-87},
doi = {10.3844/ajbbsp.2014.81.87},
url = {https://thescipub.com/abstract/ajbbsp.2014.81.87},
abstract = {Generally, bacterial culture is performed manually and is subject to error. Here, we created a novel, well-ordered and reliable system for dispensing bacteria microscopically by using paper and an ink-jet printer for controlled patterning. For paper to accommodate a culture medium, hydrophobic/hydrophilic patterns were incorporated onto the paper by immersing paper in a toluene solution of polystyrene and drying for complete hydrophobization, followed by etching discrete, small areas of hydrophilicity by ink-jet printing with toluene. Agar was hydrolyzed with sulfuric acid for appropriate viscosity and dispensed with an ink-jet printer. In a separate experiment, bacterial cells were sequentially printed on a medium and colonies were observed microscopically. The results of this experiment ensured the successful dispensing of bacteria using ink-jet printing. An almost constant number of particles per droplet were ejected using a polystyrene latex as a model of bacterial dispersion. Consequently, we expect this technology to be adapted for the development of a paper-based bioassay system.},
journal = {American Journal of Biochemistry and Biotechnology},
publisher = {Science Publications}
}