@article {10.3844/ajbbsp.2010.89.102,
article_type = {journal},
title = {A Proteomics Analysis of Drought Stress-Responsive Proteins as Biomarker for Drought-Tolerant Sugarcane Cultivars},
author = {Jangpromma, Nisachon and Kitthaisong, Supansa and Lomthaisong, Khomsorn and Daduang, Sakda and Jaisil, Prasit and Thammasirirak, Sompong},
volume = {6},
number = {2},
year = {2010},
month = {Jun},
pages = {89-102},
doi = {10.3844/ajbbsp.2010.89.102},
url = {https://thescipub.com/abstract/ajbbsp.2010.89.102},
abstract = {Problem statement: The prime objective in breeding selection process of drought-tolerant sugarcane is to identify the correlating marker, which could lead to rapid screening for drought-tolerant cultivars. In this study, we have reported an unknown 18-kDa protein (p18) along with other stress-inducible proteins to be highly expressed in sugarcane leaves under drought stress condition. Approach: The 2D-PAGE patterns of proteins were compared between those expressed in drought-tolerance K86-161 and drought-susceptible Khon Kaen 1 cultivars. The interested proteins were identified by mass spectrometry. The correlation between p18 expression and drought tolerance was verified in additional 4 sugarcane cultivars using ELISA and western blotting. Two physiological indexes, Chlorophyll content and SOD activity were also evaluated. Results: Mass spectrometry and comparison with known sequences in the database reveal that the proteins expressed only in stressed K86-161 are serine protease inhibitor and the one similar to replication protein A1. A group of proteins up-regulated in K86-161 are S-Adenosylmethionine decarboxylase proenzyme (SAM), ubiquitin and p18. From ELISA and western blotting analysis, we found that p18 expressed in drought-tolerant sugarcane cultivars had higher binding specificity to antibody than that in drought-susceptible sugarcane cultivars. Two physiological indexes showed higher levels in drought-tolerant than those in drought susceptible sugarcanes. Conclusion: These high levels of chlorophyll and SOD are in agreement with a high level of p18 expression in drought-tolerant sugarcanes. It is likely that an accumulation of p18 is a response to water deficit. In conclusion, p18 might be a good candidate for development as a marker in drought-tolerant plants.},
journal = {American Journal of Biochemistry and Biotechnology},
publisher = {Science Publications}
}