TY  - JOUR
AU  - Nurachman, Zeily 
AU  - Kurniasih, Sari Dewi 
AU  - Puspitawati, Ferra 
AU  - Hadi, Sarwono 
AU  - Radjasa, Ocky Karna 
AU  - Natalia, Dessy 
PY  - 2010
TI  - Cloning of the Endoglucanase Gene from a Bacillus amyloliquefaciens PSM 3.1 in Escherichia coli Revealed Catalytic Triad Residues Thr-His-Glu
JF  - American Journal of Biochemistry and Biotechnology
VL  - 6
IS  - 4
DO  - 10.3844/ajbbsp.2010.268.274
UR  - https://thescipub.com/abstract/ajbbsp.2010.268.274
AB  - Problem statement:  An Indonesian marine bacterial isolate, Bacillus amyloliquefaciens PSM 3.1 was isolated for hydrolyzing cellulose. A 1500-bp nucleotide fragment was amplified from the chromosomal DNA by the use of primers directed against the conserved sequence of Bacilli endoglucanase genes obtained from GenBank. Approach: The fragment was cloned and expressed in Escherichia coli. Results: The endoglucanase gene (eglII gene) had an open reading frame of 1500 nucleotides encoding a protein of 499 amino acids. The EglII protein belonged to Glycosyl Hydrolase family 5 (GH5) with a Cellulose Binding Module 3 (CBM 3). The structure model of the EglII protein revealed that the catalytic residues seemed to be Glu169 (as proton donor) and Glu257 (as nucleophile) and the catalytic triad residues were Thr256, His229 and Glu169. The EglII endoglucanase exhibited an optimum pH of 6.0 and temperature of 50°C and the enzyme tolerated to high salt concentration. Conclusion/Recommendations: This EglII endoglucanase is a promising candidate for many applications in biomass degradation.