@article {10.3844/ajbbsp.2009.137.141,
article_type = {journal},
title = {The Supervirulence Plasmid pToK47 from Agrobacterium tumefaciens A281 Improves Transformation Efficiency of Hevea brasiliensis},
author = {Arokiaraj, P. and Leelawathy, R. and Yeang, H. Y.},
volume = {5},
number = {3},
year = {2009},
month = {Sep},
pages = {137-141},
doi = {10.3844/ajbbsp.2009.137.141},
url = {https://thescipub.com/abstract/ajbbsp.2009.137.141},
abstract = {Problem statement: The present study investigates the ability 
of the pTok47 supervirulence plasmid form Agrobacterium tumefaciens A281 to enhance 
genetic transformation in Hevea brasiliensis. Approach: Hevea 
anther callus was transformed via Agrobacterium-mediated genetic transformation 
using two strains of Agrobacterium (GV2260 and GV3850) harboring the human serum 
albumin cDNA and the supervirulent plasmid pToK47 from Agrobacterium tumefaciens 
A281. The transformed callus was selected using kanamycin as the selection agent 
Results: The Agrobacterium strain GV2260 benefited from the presence 
of the supervirulence plasmid in giving a higher frequency of 7.4% transformed 
callus, 344.8% embryogenesis and 11.6% plantlet production compared to the corresponding 
strain on its own giving 0.9% transformed callus, 204.5% embryogenesis and 4.4% 
plantlet production. Similarly, Agrobacterium strain GV3850 conferred a higher 
frequency using the supervirulent plasmid, resulting in 3.5% transformed callus, 
138.5% embryogenesis and 3.5% plantlet production compared to the corresponding 
strain on its own giving 0.7% transformed callus, 137.5% embryogenesis and 9.0% 
plantlet production. These findings were confirmed by the Wilcoxon Signed Rank 
Test that compared the effectiveness of the supervirulence plasmid in increasing 
the rate of genetic transformation in the calli surviving in kanamycin growth 
medium for GV2260 (p},
journal = {American Journal of Biochemistry and Biotechnology},
publisher = {Science Publications}
}