@article {10.3844/ajbbsp.2005.212.225,
article_type = {journal},
title = {Immunohistochemical Detection of Apoptosis-Related Proteins in Gerbil Hippocampus Transient Cerebral Ischemia: Neuroprotective Effect of Pitavastatin},
author = {Himeda, Toshiki and Hayakawa, Natsumi and Tounai, Hiroko and Tanaka, Aki and Kato, Hiroyuki and Araki, Tsutomu},
volume = {1},
number = {4},
year = {2005},
month = {Dec},
pages = {212-225},
doi = {10.3844/ajbbsp.2005.212.225},
url = {https://thescipub.com/abstract/ajbbsp.2005.212.225},
abstract = {Delayed and selective neuronal damage was caused in the CA1 sector of hippocampus
following 5 min of transient cerebral ischemia in gerbils. We investigated the immunohistochemical
alterations of apoptosis-related proteins such as bcl-2α, bcl-xs/l, bax, cytochrome c, and active caspase
3 and TUNEL staining in the hippocampus at 1 and 5 hr and 1, 2, 5 and 14 days after transient
cerebral ischemia in gerbils. We also examined the effect of 3-hydroxy-3-methylglutaryl-coenzyme A
reductase inhibitor pitavastatin against the alterations of apoptosis-related proteins and TUNEL
staining in the hippocampus after cerebral ischemia. The alterations of apoptosis-related proteins in the
hippocampal CA1 sector were more pronounced than the changes of hippocampal CA3 sector and
dentate gyrus after cerebral ischemia. The alterations of apoptosis-related proteins in the hippocampal
CA1 sector after cerebral ischemia preceded the neuronal damage in this region. Furthermore, the
study with TUNEL staining showed that a marked increase of TUNEL-positive nuclei was evident
only in the hippocampal CA1 sector 5 days after cerebral ischemia. Our immunohistochemical study
also showed that pitavastatin prevented the alterations of apoptosis-related proteins and the increase of
TUNEL-positive nuclei in the hippocampal CA1 sector 5 days after cerebral ischemia. The present
study indicates that transient cerebral ischemia in gerbils causes the mitochondrial-dependent apoptosis
in the hippocampal CA1 sector. Furthermore, our present study demonstrates that pitavastatin can
prevent the alterations of apoptosis-related proteins and the increase of TUNEL-positive nuclei in the
hippocampal CA1 sector after cerebral ischemia. Thus our study provides novel therapeutic strategies
in clinical stroke.},
journal = {American Journal of Biochemistry and Biotechnology},
publisher = {Science Publications}
}