TY  - JOUR
AU  - Amanah, Rafika Mila 
AU  - Putri, Dita Prameswari Trenggono 
AU  - Abidin, Mohammad Zainal 
AU  - Erwanto, Yuny 
PY  - 2025
TI  - Isolation and Characterization of Collagen from IndonesiaLocal Duck Feet Using Bromelain Enzyme
JF  - American Journal of Animal and Veterinary Sciences
VL  - 20
IS  - 2
DO  - 10.3844/ajavsp.2025.97.102
UR  - https://thescipub.com/abstract/ajavsp.2025.97.102
AB  - The utilization of local duck feet as a collagen source to minimizewaste and add value to the duck by-product. This research aimed to isolate andcharacterize collagen from Local duck feet using enzymatic method. Thebromelain enzyme in various concentrations: 0% (A), 0.2% (B), 0.4%(C), 0.6%(D), and 0.8% (E). Variables observed were collagen yield, viscosity, pH,Fourier Transform Infrared Spectroscopy (FTIR), and Sodium Dodecyl SulfatePolyacrylamide Gel Electrophoresis (SDS-PAGE). The results showed the highestcollagen yield was 7.77% from D treatment; the viscosity ranges were 2.18-1.99cP, and the pH ranges were 4.89-4.68. FTIR spectra showed amide A, B, I, II,and III. SDS-PAGE results showed three distinct main bands, consisting of α1,α2, and β chains. In conclusion, collagen could be extracted from Local duckfeet and categorized as collagen type I.