@article {10.3844/ajavsp.2025.26.40, article_type = {journal}, title = {Biosynthesis and Evaluation of Zinc, Copper, and Methionine Feed Supplements Using Saccharomyces cerevisiae on Duck Performance in Minimal Water Systems}, author = {Setiyatwan, Hendi and Ismiraj, Muhammad Rifqi}, volume = {20}, number = {1}, year = {2025}, month = {Feb}, pages = {26-40}, doi = {10.3844/ajavsp.2025.26.40}, url = {https://thescipub.com/abstract/ajavsp.2025.26.40}, abstract = {Optimizing duck performance under minimal water conditions is essential for sustainable poultry farming. Biosynthesizing essential feed supplements like Zinc (Zn), Copper (Cu), and methionine using Saccharomyces cerevisiae presents a promising method to enhance growth and meat quality. This study developed a biosynthesis method for Zn, Cu, and methionine supplements using S. cerevisiae and evaluated their effects on duck performance. In experiment 1, substrates containing ZnO, CuSO₄, and tryptophan were fermented with varying concentrations of S. cerevisiae spores (1×10⁷, 2×10⁷ and 3×10⁷ spores/100 g) for 3, 5, 7, or 9 days. The fermentation process was assessed for physical, chemical, and biological quality to determine optimal conditions. The optimal condition was identified as 3×10⁷ spores/100 g substrate with a 9-day fermentation period, resulting in significant increases in Zn content by 25% (p}, journal = {American Journal of Animal and Veterinary Sciences}, publisher = {Science Publications} }