@article {10.3844/ajavsp.2024.172.182,
article_type = {journal},
title = {Quality of Sperm Simmental Bulls and Success of Artificial Insemination with the Addition of Nanocalcium Phosphate in Tris Aminomethane Egg Yolk Diluent Using Semen Storage Ampoules from Nanocalcium Silicophosphate Biomaterials},
author = {Kusumawati, Enike Dwi and Karyasa, I Wayan and Putra, Yogi Pratama and Kari, Asmad and Komilus, Connie Fay},
volume = {19},
number = {2},
year = {2024},
month = {May},
pages = {172-182},
doi = {10.3844/ajavsp.2024.172.182},
url = {https://thescipub.com/abstract/ajavsp.2024.172.182},
abstract = {Success in Artificial Insemination (AI) is largely determined by the quality of the stored semen, therefore storage devices, especially straws or ampoules, are an important part that cannot be separated from the overall semen cryopreservation technology. Making a prototype of semen storage ampoules from nanocalcium silicophosphate biomaterials is based on the need for straws or ampoules. Semen storage in spermatozoa cryopreservation technology for the needs of AI and livestock breeding and from the results of an examination of previously patented inventions. The aim of this research was to determine the quality of simental cow sperm and the success of AI with the addition of nanocalcium phosphate to the Tris aminomethane egg yolk diluent using semen storage ampoules made from nanocalcium silicophosphate biomaterial. This research method uses laboratory research with a factorial Completely Randomized Design (CRD) consisting of 2 factors, namely the diluent factor consisting of 2 treatments (tris aminomethane egg yolk and tris aminomethane egg yolk + phosphate 1% w/v) and the shelf life factor. Consisting of 5 treatments (0, 1, 2, 3, 4 days). The second factor is the use of ampoules and without ampoules. Each treatment was repeated 10 times. The results of the research showed that the addition of nanocalcium phosphate to the egg yolk tris aminomethane diluent using semen storage ampoules made from nanocalcium silicophosphate biomaterial had a very significant effect (p},
journal = {American Journal of Animal and Veterinary Sciences},
publisher = {Science Publications}
}